Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria英文文献.pdfVIP

Gene, 101 (1990) 33-44 0 1991 Elsevier Science Publishers B.V. 0378-1119/91/$03.50 33 GENE 03995 Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria (Fimbriae; pili; nucleotide sequence; nucleotide-binding protein; recombinant DNA; DNA uptake) Cynthia B. Whitchurch”, Matthew Hobbs”, Susan P. Livingstona, Viji Krishnapillaib and John S. Mattick” a Centrefor Molecular Biology and Biotechnology, The Universityof Queensland, Brisbane, Qld. 4072 (Australia); and bDepartment of Genetics and Developmental Biology, Monash University, Clayton, Vie. 3168 (Australia) Tel. (61-3)5653853 Received by P.A. Manning: 9 October 1990 Revised: 19 November 1990 Accepted: 20 November 1990 SUMMARY Type-4 timbriae (pili) are associated with a phenomenon known as twitching motility, which appears to be involved with bacterial translocation across solid surfaces. Pseudomonas aeruginosu mutants which produce fimbriae, but which have lost the twitching motility function, display altered colony morphology and resistance to limbrial-specific bacteriophage. We have used phenotypic complementation of such mutants to isolate a region of DNA involved in twitching motility. This region was physically mapped to a SpeI fragment around 20 min on the P. aeruginosu PA0