Expression of thermostable bacterial b glucosidase BglB in transgenic tobacco plants英文文献.pdfVIP

Bioresource Technology 101 (2010) 7144–7150 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier .com/locate/biortech Expression of thermostable bacterial b-glucosidase (BglB) in transgenic tobacco plants Sera Jung a, Suyeon Kim b, Hanhong Bae c, Hyoun-Sub Lim d, Hyeun-Jong Bae a,b,e,* a Department of Forest Products and Technology (BK21 Program), Chonnam National University, Gwangju 500-757, Republic of Korea b Bio-Energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea c School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea d USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA e Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea a r t i c l e i n f o a b s t r a c t Article history: Transgenic tobacco plants expressing the hyperthermostable b-glucosidase BglB of Thermotoga maritima Received 18 January 2010 were generated with the goal of cost-effective production of the enzyme for the application in bioconver- Received in revised form 27 March 2010 sion of lignocellulosic biomass. The enzyme was targeted to the cytosol and chloroplasts, where it accu- Accepted 31 March 2010 mulated to level of 4.5% and 5.8% of total soluble protein, respectively. The optimal temperature and pH Available online 27 April 2010 of the plant-expressed BglB was 80 C and 4.5, respectively. BglB activity was preserved in