[自然科学]岛津GCMS培训教材.pptVIP

In QP type mass spectrometer, ions are separated in quadrupole rods. Quadrupole rods are consists of four rods and the voltage is applied to each rod.（電圧のかけ方の絵） If the voltage V1 is applied to rod, ions with m1 can pass through but the ions with other mass go off course. So we can pass　 the ions with desired mass 　by applying appropriate voltage to rods If we varies the voltage continuously, the passing mass varies accordingly and we can get mass spectrum. MS has the two data acquisition mode, one is scan mode and the other is SIM mode. As explained before, When V is continuously changed, the ions enter the detector in sequence of mass . We call the changing range “Scan range” In this example , the scan range is from 35 amu to 350 amu . We can get the mass spectrum from 35 amu to 350 amu from this measurement. The spectrum is usually measured every 0.5 to 1.0 seconds. We can deduce some chromatogram from the scan data. One is “mass chromatogram” This chromatogram is drawn using the necessary mass numbers data out of scan data. Peaks are resolved into single components or a combination of components. From mass chromatogram, (1) The detected ions are resolved as noise or signal (2)Target components are elucidated from multiple peaks Quantitation is easily performed. We call the summation of mass chromatogram over whole mass “ TIC total ion chromatogram”. TIC approximately corresponds to chromatogram of GC. Another acquisition mode is SIM (selected ion monitoring). In this mode, the voltage is not continuously varied, but is set at only desired mass numbers. This mode is used primarily in quantitative analysis. We can get higher sensitivity than mass chromatogram of scan mode because of longer period measurement for remarkable masses Selection of the mass of target compound is very important with SIM method. High sensitivity analysis is possible depending on the ion selected. （時間があればS/N) I’ve been talking about GCMS, but there’s