棉花航天诱变芽黄突变体蛋白组学分析课件.pdfVIP

花 学 报 Cotton Science 20 13 25 渊4 冤院345~351 花航天诱变芽黄突变体蛋白组学分析 * * 海晶 蒋 博 范术丽 庞朝友 宋明梅 宋美珍 喻树迅 ( 中国农业科学院棉花研究所/ 棉花生物学国家重点实验室 河南安阳455000) 摘要院以 棉所58 及其航天诱变芽黄突变体 棉所58 倒2 叶为材料 利用等电聚焦和第二向SDS 技术获得棉花叶片总蛋白图 通过ImageMaster-2D Elite 7.0 分析软件分析各个差异蛋白在两种叶片 的相对 表达量 并进行MALDI-TOF/TOF 鉴定遥 结果表明 从中棉所58 及其突变体的双向电泳图谱中共检测到41 个 差异蛋白点这些差异蛋白 点的等电点分布集中在4.0 7.0 之间 分子量分布集中在15.0 95.0 kD 之间 进一步 谱分析鉴定后获得了14 个差异蛋白点 包括核酮糖-1,5 二磷酸羧化酶/ 加氧酶尧S- 腺苷甲硫氨酸合 成酶尧黄烷酮3- 羟化酶等多种蛋白 涉及到光合作用和光呼吸尧乙烯和多胺的合成尧类黄酮的合成等生物代谢 途径遥 关键词院棉花曰芽黄突变体曰蛋白质组曰 谱分析 中图分类号院S562.035.2 文献标志码院A 文章编号院1002-7807(2013)04-0345-07 * * LI Hai-jing, JIANG Bo, FAN Shu-li, PANG Chao-you, SONG Ming-mei, SONG Mei-zhen , YU Shu-xun ( 455000, ) We extracted total protein from the second-to-top leaves from the cotton line CCRI 58 and its virescent mutant CCRI 58 , which was obtained by space mutation. The protein profiles of the two lines were obtained by isoelectric focusing fol- lowed by SDS. We analyzed the relative abundance of differentially expressed proteins in leaves between CCRI 58 and CCRI 58 using ImageMaster-2D Elite 7.0 software, and further identified the differentially expressed protein spots by ma- trix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF) analysis. The results showed that 4 1 proteins showed differential expressions between CCRI 58 and CCRI 58 . The PI values of these proteins were mainly con- centrated in the range of 4.0 to 7.0 and their molecular weights ranged from 15.0 to 95.0 kDa. After further MALDI-TOF/TOF analysis, we identified 14 of t