棉花转录因子GhWRKY基因的克隆及特征分析精品.pdfVIP

花 学 报 Cotton Science 20 13 25 渊3 冤院205~210 花转录因子 4 基因的克隆及特征分析 光雷 范术丽 宋美珍 庞朝友 魏恒玲 喻树迅* ( 中国农业科学院棉花研究所/ 棉花生物学国家重点实验室 河南安阳455000) 摘要院本研究从陆地棉中克隆得到 4 基因 全长cDNA 是1281 bp 包含 1083 bp 的开放阅读框 编码 的360 个氨基酸属于IIcWRKY 转录因子家族遥 分析 4 基因结构表明其有三个外显子和两个内含 子遥 GhWRKY4 蛋白的亚细胞定位实验表明其定位在洋葱表皮细胞核遥 实时荧光定量分析结果显示 4 在棉花的根尧茎尧叶和花中均有表达遥 染色体步移得到了 4 的5 爷端侧翼序列 生物信息学软 件预测表明 4 包含一系列和胁迫基因调控相关的反式作用元件 表明 4 参与植物对盐害和 冷害胁迫的反应遥 关键词院 4 基因曰棉花曰转录因子曰胁迫 中图分类号院S562.035.3 文献标志码院A 文章编号院1002-7807(2013)03-0205-06 LI Guang-lei, FAN Shu-li, SONG Mei-zhen, PANG Chao-you, YU Shu-xun* ( 455000, ) In the present study, a WRKY gene, 4, was cloned from cotton( L.). The full-length cD- NA of 4 was 1281 bp long and contained a 1083-bp open reading frame (ORF) encoding a putative group-IIc WRKY protein of 360 amino acids. Bioinformatic analysis of the 4 gene revealed that 4 consists of three exons and two introns. A subcellular localization assay confirmed that the protein product was targeted in onion epidermal cell nuclei. Quantitative RT-PCR indicated that 4 is constitutively expressed in cotton roots, stems, leaves, and flowers. The 5 flanking region of 4 was obtained by genome walking. Bioinformatic analysis revealed that 4 contains a se- ries of putative -acting elements involved in stress regulation. Further stress treatments combined with gene expression analy- sis indicated that 4 is involved in regulation of salt and cold stress. 4 gene; L.; transcription factor; stress response WRKY 转录因子是植物中最大的转录因子 量(I 组蛋白 1 个WRKY 结构域和另外