Movementof Cations Cuticles Citrus aurantium Plant Physiol运动的阳离子角质层枳实植物生理.pdfVIP

Plant Physiol. (1990)94, 120-126 Received for publication February 13,1990 0032-0889/90/94/01 20/07/$01 .00/0 Accepted April 21, 1990 Movement of Cations through Cuticles of Citrus aurantium and Acer saccharum1 Diffusion Potentials in Mixed Salt Solutions Melvin T. Tyree*, Christopher A. Tabor, and Charles R. Wescott Northeastern Forest Experiment Station, P. 0. Box 968, Burlington, Vermont 05402 (M.T. T., C.A. T., C.R. W.), Botany Department (M.T. T.) andAgricultural Biochemistry Department (C.R.W.), University of Vermont, Burlington, Vermont 05405 ABSTRACT and hypothesized a charged-pore model that explains the We examined some biophysical mechanisms of ion migration asymmetric properties ofcuticles reflected by diffusion poten- tials across cuticles ofAcer and Citrus. That work was based across leaf cuticles enzymatically isolated from Acer saccharum L. and Citrus aurantium L. leaves. Diffusion potential measure- exclusively upon diffusion potentials generated by using KCI ments were used to calculate the permeabilities of Cl-, Li+, Na+, solutions, and all experiments were conductedwith salt-bridge and Cs+ ions all as a ratio with respect to the permeability of K+ electrodes of a type traditionally used in electrophysiological in cuticles. In 2 millimolar ionic strength solutions the permeability studies, i.e. by embedding Ag-AgCl wires in a KCl-gel (aga- sequence from high to low was K = Cs Na Li Cl. When the ro