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Biochemical Systematics and Ecology 43 (2012) 73–79 Contents lists available at SciVerse ScienceDirect Biochemical Systematics and Ecology journal homepage: www.elsevi /locate/biochemsyseco Assessment of genetic diversity in Achillea millefolium accessions from Iran using ISSR marker Mostafa Farajpour a, Mohsen Ebrahimi a, *, Reza Amiri a, Rasoul Golzari b, Sepideh Sanjari a a Department of Agronomy and Plant Breeding, College of Abouraihan, University of Tehran, Tehran, Iran b Department of Agronomy and Plant Breeding, College of Agriculture, University of Zanjan, Zanjan, Iran a r t i c l e i n f o a b s t r a c t Article history: Thirty-seven accessions of Achillea millefolium collected from provinces in Iran, were Received 20 April 2011 analyzed using inter-simple -sequence -repeat (ISSR) markers to evaluate their variability. Accepted 19 February 2012 A total of seven ISSR primers generated 72 amplified fragments, most of which were Available online 28 March 2012 polymorphic. The Jaccard similarity indices, as based on the ISSR profiles, were subjected to a complete linkage analysis, and the dendrogram revealed six groups. The results of the Keywords: clustering showed that A . millefolium subspecies elbursensis (endemic to Northern Iran) is Genetic diversity separated from other genotypes in the dendrogram. The Principle Component Analysis Achillea millefolium ISSR marker