Assessment of genetic diversity in castor (Ricinus communis L.) using RAPD and ISSR markers推荐.pdfVIP

Industrial Crops and Products 32 (2010) 491–498 Contents lists available at ScienceDirect Industrial Crops and Products j o u rn al h omepage: w w w. elsev /locate/indcrop Assessment of genetic diversity in castor (Ricinus communis L.) using RAPD and ISSR markers Bhavesh B. Gajera a , Nitish Kumar a , Amritpal S. Singh a,∗ , Bhupendra Singh Punvar a , R. Ravikiran a , N. Subhash a , G.C. Jadeja b a Department of Agricultural Biotechnology, Anand Agricultural University, Anand 388110, Gujarat, India b Department of Agricultural Botany, Anand Agricultural University, Anand 388110, Gujarat, India a r t i c l e i n f o a b s t r a c t Article history: Castor (Ricinus communis L.) is an important plant for production of industrial oil. The systematic evalua- Received 3 March 2010 tion of the molecular diversity encompassed in castor inbreds or parental lines offers an efficient means Received in revised form 18 June 2010 of exploiting the heterosis in castor as well as for management of biodiversity. Two DNA-based molecular Accepted 21 June 2010 marker techniques, viz., random amplified polymorphism DNA (RAPD) and inter simple sequence repeat (ISSR), were used to assess the genetic diversity in castor genotypes. Out of the 200 RAPD and 21 ISSR primers screened, a total of 35 polymorphic primers (30 RAPDs and 5 ISSRs), were used in this study. Keywords: Amplification of genomic DNA