Assessment of genetic diversity and structure of Imperial Crown推荐.pdfVIP

Biochemical Systematics and Ecology 42 (2012) 35–48 Contents lists available at SciVerse ScienceDirect Biochemical Systematics and Ecology journal homepage: www.elsevi /locate/biochemsyseco Assessment of genetic diversity and structure of Imperial Crown (Fritillaria imperialis L.) populations in the Zagros region of Iran using AFLP, ISSR and RAPD markers and implications for its conservation Sajad Badfar-Chaleshtori a, b, Behrouz Shiran a, c, *, Masoomeh Kohgard a, Hassan Mommeni a, Akram Hafizi a, Mahmood Khodambashi a, Neda Mirakhorli a, Karim Sorkheh a a Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, PO Box 115, Iran b Department of Agronomy and Plant Biotechnology, Faculty of Agriculture, Payame Nor-e-Tehran University, Tehran, PO Box, Iran c Institute of Biotechnology, Shahrekord University, Shahrekord, PO Box 115, Iran a r t i c l e i n f o a b s t r a c t Article history: Fritillaria imperialis grows at high altitudes of the alpine Zagros region of Iran and has Received 2 August 2011 a high medicinal and ornamental value and importance. In this study AFLP, ISSR and RAPD Accepted 28 December 2011 markers were used to detect intra- and inter-population genetic diversity of F. imperialis. Available online 9 March 2012 140 genotypes, belonging to seven populations, were studied using six random primers, six ISSR primers and five AFLP primer combinations, to investigate the genetic structure Keywords: between popu