外阴阴道假丝酵母菌病患者阴道分泌物中肺表面.doc

SP-A在RVVC患者阴道分泌物中的表达及意义 明燕虹，袁瑞重庆医科大学附属第一医院妇产科，重庆 400016 摘要：目的 探讨肺表面活性物质相关蛋白A(SP-A)在复发性外阴阴道假丝酵母菌病(RVVC)患者阴道分泌物中的表达及意义。方法 收集RVVC患者、外阴阴道假丝酵母菌患者(VVC)及健康体检者（对照组）阴道分泌物各20例，用酶联免疫吸附测定法(ELISA)测定各组中SP-A蛋白的含量,用逆转录聚合酶链式反应(RT-PCR)方法检测各组中SP-A的基因表达情况。SP-A蛋白的含量发现：VVC组的SP-A明显高于对照组（P0.05)， RVVC组的SP-A高于对照组及VVC组（P0.05)。RT-PCR方法发现：VVC组的SP-AmRNA的表达水平高于对照组（P0.05)，RVVC组的SP-AmRNA的表达水平明显高于对照组及VVC组（P0.05) SP-A可能是RVVC患者抗假丝酵母菌的重要物质，其性质或数量的改变可能在RVVC的发病机制中发挥重要作用。 关键词：念珠菌病，外阴阴道；肺表面活性物质相关蛋白A；基因表达 Expression and signification of pulmonary surfactant-associated protein-A in the vaginal fluid of vulvovaginal candidiasis MING Yan-Hong,YUAN Rui(Department of Obstetrics and Gynecology,the Affiliated First Hospital of Chongqing Medical University,Chongqing,400016,China) Abstract:Objective To study the expression and signification of pulmonary surfactant-associated protein-A in the vaginal fluid of recurrent vulvovaginal candidiasis(RVVC).Methods We collected human vaginal fluid from 20 RVVC patients,20 VVC patients and 20 healthy women(the control group).Enzyme-linked immunosorbent assay(ELISA) was applied to detect the levels of SP-A.The expression of SP-A gene was detected by reverse transcription–polymerase chain reaction (RT–PCR).Results In contrast to the control group,the levels and of SP-A of VVC group were higher,which was considered to be significant ( P 0. 05).The levels of SP-A of recurrent vulvovaginal candidiasis (RVVC) group were higher than those of VVC group and control group.The expression of SP-A mRNA in VVC was higher than that in the control group.The expression of SP-A mRNA in RVVC was significantly higher than those of VVC group and control group.Conclusion SP-A might be an essential component in RVVC resistance. The qualitative and quantitative alterations of normal SP-A may play a role in the pathogenesis of RVVC. Keywords: Vulvovaginal candidiasis;Pulmonary surfactant-associated protein-A; Gene expression 复发性外阴阴道假丝酵母菌病(recurrent vulvovaginal