【医学课件】 Environmental Sampling.pptVIP

Practical 12Environmental Sampling Microbiological Sampling of Air Environmental Sampling Environmental microbiology is not clinical microbiology(Air, water , soil, food , etc.) Random, undirected sampling is not recommended Sampling requires a protocol for sampling and culturing, analysis of results, and action based on the interpretation of results General Environmental Sampling Suggested uses: Outbreak investigation Research in Environmental infection control Monitor a potentially hazardous situation Evaluate a change in environmental infection control for quality purposes Periodic maintenance of equipment HAC systems ( heating air conditioning system) Legal issues Environmental Sampling Problems Expensive Time-consuming Subject to many variables in protocol, analysis, and interpretation Why difficult and problematic No baselines, no acceptable ranges Few protocols for conducting planned, directed environmental studies in health care settings. The investigator is required to minimize false negatives and false positives. Air Sampling Why do Air Sampling? Verification of ventilation and cleanliness Establish baseline data Post infection evaluation (outbreak investigation) Rule out ventilation as a source Discover source of infectious fungi (reservoir) Construction, renovation, repair of certain buildings such as hospitals Employee complaints Microorganisms of the air Important Facts: Air has no indigenous or native flora Organisms are found temporarily suspended in air or carried on dust particles or droplets Air is not sterile Air does not support the growth of organisms Before Microbiological Air Sampling… Define your objective and analytical approach Qualitative vs. quantitative Compare indoor results to counts from outdoor air Fully describe the circumstances in the area where sampling is occurring High volume sampling most efficient Poor Ventilation Inadequate ventilation can increase indoor pollutant levels by not bringing in enough outdoor