【医学课件】 Escherichia coli.pptVIP

Corynebacterium diphtheriae Microscopic examination * Gram +ve rods * pleomorphic (Chinese letter,V, palisades arrangment) * Non spore forming * non motile * non capsulated * Metachromatic granules (volutin granules) * Blue biplar with albert stain Corynebacterium diphtheriae is a pathogenic bacterium that causes diphtheria. It is also known as the Klebs-L?ffler bacillus Macroscopic appearance Cultural appearance Black colony on tellurite blood agar (Tinsdales agar) due to the production of H2S from cystine biochemical test Gelatin -ve Oxidase +ve Starch +ve Glucose +ve Maltose +ve Bacillus anthracis Bacillus anthracis is a pathogenic bacterium that causes anthrax disease Microscopic examination * Gram +ve rods Arranged in long chains with squre ends aerobic Oval central spore , not bulging Capsulated Non motile Macroscopic appearance Cultural appearance ?-hemolysis on blood agar Medusa head on nutreint agar   biochemical test Gelatin +ve Catalase +ve Medusa head of bacillus anthracis Bacillus subtilis Microscopic examination Gram +ve rods blunt ends Oval central or subterminal spore,bulging Motile Non Capsulated  Macroscopic appearanceCultural appearance β- hemolysis on blood agar Medusa head on nutreint agar biochemical test Gelatin +ve Catalase +ve Clostridium Cl. tetani Tetanus Cl. Perfringens Gas gangrene Cl. botulinum Botulism Cl. difficille Antibiotic associated diarrhea Cl. Perfringens Cl. Perfringens( C. welchii ) is a pathogenic bacterium that causes Gas gangrene and Food poisoning (Enterotoxin) Microscopic appearance Large Gram-positive bacilli with blunt ends The spore is oval, sub-terminal non bulging Spores are rarely observed Anaerobic Capsulated Non motile Macroscopic appearanceCultural appearance On blood agar → ? double zone of hemolysis on blood agar in cooked meat medium (prepared from heart muscles) which contain hematin glutathione that act as reducing agent → pink due to saccharol