生物信息学-第三章-生物信息数据库及其信息检索.pptVIP

Exon：region of genome that codes for portion of spliced mRNA, rRNA and tRNA, may contain 5`UTR, all CDSs and 3`UTR. Intron：a segment of DNA that is transcribed, but removed from within the transcript by splicing together the sequence (exons) on either side of it. Exon 1 Exon 3 I I I I Exon 2 5`UTR 3`UTR Splicing Exon 1 Exon 3 Exon 2 Exon 1 Exon 3 Exon 2 Exon 1 Exon 2 Exon 3 Exon 2 Exon 1 Exon 3 Misc-binding：site in nucleic acid which covalently or non-covalently binds another moiety that cannot be described by any other binding key (primer-bind or protein-bind). Misc-feature：region of biological interest which cannot be described by any other feature key, a new or rate feature. Misc-RNA：any transcript or RNA product that cannot be defined by other RNA keys (prim-transcript, precursor-RNA, mRNA, 5`clip, 3`clip, 5`UTR, 3`UTR, exon, CDS, sig-peptide, transit-peptide, mat-peptide, intron, polyA-site, rRNA, tRNA, scRNA and snRNA). Misc-signal：any region containing a signal controlling or altering gene function or expression that cannot be described by other signal keys (promoter, CAAT-signal, TATA-signal, -35-signal, -10-signal, GC-signal, RBS, polyA-signal, enhancer, attenuator, terminator, and rep-origin). Stem-loop：hairpin, a double-helical region formed by base-pairing between adjacent (inverted) complementary sequences in a single strand of RNA or DNA. 5`TGGAATTGTGAGCGGATAACAATT 3`ACCTTAACACTCGCCTATTGTTAA 5` 3` T A G C G C A T A T T A T A G C T A G A G C G G A T A T T A A C A A A T T G T C G T T C C C T A Stem-loop Stem-loop 3` 5` 北京华大基因研究中心(中科院基因组研究所) 杨焕明 / 国家人类基因组南方研究中心(上海) 陈竺、赵国屏 / 国家人类基因组北方研究中心(北京) 强伯勤 / 清华大学生物系生物信息研究室 孙之荣 / 北京大学生物信息学中心 罗静初 /chinese/ 复旦大学理论生物中心 钟扬 / 我国的一些主要研究中心和数据库 生物信息学数据库的检索 检索方法概述 检索实践和案例 生物信息学数据库的检索 主要检索系统和工具 Entrez(National Center for Biotechnology Information, NCBI） SRS(European Bioinformatics Institute, EBI） ExPasy Expert Protein Analysis System (Swiss Institute of Bioinformatics, SIB) 日本、欧洲、美国其他研究机构的工具平台…… 复杂检索 1、限制字段类别 常用的有: Author: