喷施糠氨基嘌呤对铝胁迫大麦幼苗的缓解效应-浙江大学学报.PDFVIP

浙江大学学报 （农业与生命科学版）38(6) ：709~714，2012 Journal of Zhejiang University (Agric. Life Sci.) /agr E-mail: zdxbnsb@ DOI: 10.3785/j.issn.1008-9209.2012.04.231 喷施 6-糠氨基嘌呤对铝胁迫大麦幼苗的缓解效应 * 郭天荣 ，潘伟槐 （绍兴文理学院生命科学学院，浙江 绍兴 312000 ） 摘要 采用营养液培养试验，研究叶面喷施不同浓度6-糠氨基嘌呤 (6-KT) (100 、200、400和800 μmol/L) 对铝胁 迫大麦幼苗生长、叶片叶绿素含量、游离脯氨酸含量、活性氧清除酶活性的影响. 结果表明：与单独铝胁迫相比， 喷施100~400 μmol/L 6-KT 可改善大麦幼苗生长，提高叶绿素含量，降低叶片丙二醛 (malondialdehyde, MDA) 和脯 氨酸含量，降低叶片过氧化物酶 (peroxidase, POD) 和超氧化物歧化酶 (superoxide dismutase, SOD) 活性；喷施200 和400 μmol/L 6-KT 亦可降低过氧化氢酶 (catalase, CAT) 活性；当喷施200 μmol/L 6-KT 时各指标的增幅最大，与对 照组值最接近，但当喷施浓度为800 μmol/L 时植株生物量、叶绿素含量下降，而MDA 、脯氨酸含量和抗氧化酶活 性上升. 表明适宜浓度的外源6-KT可有效缓解大麦由于铝毒害引发的生长抑制和氧化胁迫，但浓度过高则产生氧 化作用. 关键词 6-糠氨基嘌呤 (6-KT) ；铝胁迫；大麦幼苗；生长；抗氧化能力 中图分类号 Q 945. 78; S 311 文献标志码 A Alleviating effect of 6-kinetin spraying on aluminum toxicity in barley seedlings. Journal of Zhejiang University (Agric. Life Sci.), 2012, 38(6): 709-714 * GUO Tian-rong , PAN Wei-huai (College of Life Sciences, Shaoxing University, Shaoxing, Zhejiang 312000, China) Summary Aluminum (Al) is considered as the major factor constraining crop performance when soil pH is lower than 5.5. The most easily recognized symptom of A1 toxicity is the inhibition of root growth, and this has become a widely accepted measure of A1 stress in plants. In simple nutrient solutions, micromolar concentrations of A1 can begin to inhibit root growth within 60 min. Kinetin (KT), one of the cytokins, is a unique plant hormone that promotes cell division. KT has been widely used to alleviate the toxicity in plants caused by abiotic stresses, fo