(毕业论文)铅与Vc对蛋仔鸡脾脏脂质过氧化的影响.docVIP

铅与Vc对蛋仔鸡脾脏脂质过氧化的影响 毕业生：魏周文 指导教师：孙超 西北农林科技大学动物科技学院 陕西 杨凌 712100 【摘要】 目的 探讨铅与Vc对鸡脾脏过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、超氧化物岐化酶(SOD)活性、以及丙二醛(MDA)含量的影响。方法 蛋仔鸡以不同浓度（分别为0.02mg/g体重、0.05mg/g体重、0.08mg/g体重、0.11mg/g体重）灌注醋酸铅以及Vc与醋酸铅配伍， 饲喂10天后测定脾脏POD活性、CAT活性、SOD活性、MDA含量。结果 POD活性随铅浓度增加而降低，与对照组相比，铅染毒组和Vc+铅染毒组差异都显著（P0.05），但铅染毒组四个水平下差异不显著（P0.05），Vc+铅四个水平差异显著（P0.05），同浓度下加Vc后比不加Vc的活性高，差异不显著（P0.05）。CAT活性随着剂量的加大染毒组较对照组低且有下降趋势，并且与普通对照组比较差异显著（P0.05），铅染毒组内差异显著（P0.05），Vc+铅染毒组内差异不显著（P0.05），但同浓度下加Vc后比不加Vc的活性高，除0.11mg/g体重染毒组差异显著（P0.05）外其余组差异不显著（P0.05）。随铅浓度升高SOD活性下降，与对照组比较差异显著（P0.05），各组组内差异不显著（P0.05），同浓度下加Vc后比不加Vc的活性高，差异不显著（P0.05）。MDA含量随铅浓度增加而增加，较普通对照组差异显著（P0.05），加入Vc后虽有变化，但差异不显著P0.05，铅染毒组组内差异显著（P0.05），铅+Vc染毒组差异不显著P0.05，同浓度下加Vc后比不加Vc的含量低，差异不显著（P0.05）。结论 铅可引起蛋仔鸡脾脏POD、CAT、SOD活性以及MDA含量的改变，表明铅可引起体内氧应激反应导致脂质过氧化损伤，但Vc可减轻脂质过氧化损伤。 【关键词】 铅，Vc, 蛋仔鸡，脂质过氧化，脾脏 Abstract: Objective To explore the effect lead takes to MDA POD CAT SOD in chickens’ spleen. Methods Chickens were exposed to lead by drinking lead acetate solution and lead acetate and vitamin C solution everyday. Each has 4 different levels: 0.02 microgram per one gram weight,0.05 microgram per one gram weight ,0.08 microgram per one gram weight, 0.11 microgram per one gram weight . After 10 days, activities of CAT SOD POD and concentration levels of MDA were measured. Results Compared with the control group POD activity in every group lowered significantly (P0.05), but lead groups had no difference (P0.05). Vc and lead groups had significantly difference (P0.05).At the same level, Vc and lead groups were not significantly higher than which hadn’t Vc(P0.05). CAT activity in every group lowered significantly (P0.05) compared with the control group（P0.05）,lead groups had significant difference(P0.05). Vc and lead groups had no difference（P0.05）. But the same level, Vc and lead groups were not significantly higher than which hadn’t Vc(P0.05) except 0.11mg/g weight group. SOD activity were significantly lower than control group(P0.05).It had no difference amo