子痫前期患者胎盘组织中iex-1的表达及其意义.docVIP

子痫前期患者胎盘组织中IEX-1的表达 耿莉娜,韩丽萍*，张晓雪 (郑州大学第一附属医院妇产科 郑州450052) 【摘要】目的：检测子痫前期患者胎盘组织中即刻早期反应基因X-1（IEX-1）mRNA30例子痫前期患者（PE组，轻度组10例、重度组20例）和20例正常足月孕妇 (对照组),取其胎盘中央母体面组织，应用RT-PCR和免疫组化SP法检测各组胎盘组织中IEX-1mRNA和蛋白的表达水平。结果: ①.IEX-1mRNA相对表达量为:对照组(0.874±0.099),轻度PE组(1.270±0.104)，重度PE组(1.980±0.117),3组比较差异有显著性（F=537.296，P0.001）,且随病情严重程度的增加而升高。②.IEX-1主要表达于胎盘滋养细胞。子痫前期组IEX-1的阳性表达率：重度PE组95%(19/20)，轻度PE组60%(6/10)，两组比较差异有统计学意义(χ2=21.290, P0.001)；正常妊娠组IEX-1的阳性表达率20%(4/20)，与子痫前期两组比较，差异均有统计学意义（P0.01)；且蛋白表达水平与mRNA水平相一致。结论:IEX-1可能通过调节滋养细胞凋亡参与子痫前期的病理生理过程。 关键词：即刻早期反应基因X-1；子痫前期；胎盘 Expression of IEX-1 in pre-eclampsia placental tissue GENG Li-na，HAN i-ping*， ZHANG iao-xue Department of Obstetrics and Gynecology，the First Affiliated Hospital，Zhengzhou University，Zhengzhou,450052, China. [Abstract] Aim: To examine the mRNA and protein expressions of immediate early response gene X-1 (IEX-1) in pre-eclampsia placenta, and to explore its relationship with the pathogenesis of pre-eclampsia. Methods: Thirty patients with preeclampsia (PE group, mild group 10 cases, and severe group 20 cases) and 20 cases of normal pregnant women (control group) were randomly obtained. Samples were taken from the central maternal placental tissue. We detected IEX-1 mRNA and protein expressions in placenta using RT-PCR and immunohistochemistry. Results: ①.IEX-1 mRNA relative expression: control group (0.874±0.099), mild PE group (1.270±0.104), severe PE group (1.980±0.117)。 the results between these three groups: There are differences among the three groups(F=537.296，P0.001).IEX-1 mRNA expression increases in with the severity of the disease.②. IEX-1 was mainly expressed in placental trophoblast cells.IEX-1 protein positive expression: in severe preeclampsia group, the positive rate was 95% (19/20); and in mild pre-eclampsia group, the positive expression rate was 60% (6/10); The difference between two group was statistically significant (χ2=21.290, P0.001). The positive rate from normal pregnancy group was 20% (4/20); Respecti