生防细菌SY286的筛选及其对葡萄霜霉病的防治效果研究-无机材料学报.PDF

30(3)402-407 中国生物防治学报 Chinese Journal of Biological Control 2014 年 6 月 生防细菌SY286的筛选及其对葡萄霜霉病的防治效果研究 1,2 2* 2 2 2 2 臧超群 ，赵奎华 ，刘长远 ，梁春浩 ，于舒怡 ，刘 丽 （1. 沈阳农业大学植物保护学院，沈阳 110866 ；2. 辽宁省农业科学院植物保护研究所，沈阳 1 10161 ） 摘要：从辽宁省 9 个地区葡萄园采集葡萄叶片 36 份，分离获得 303 株细菌，并筛选出对葡萄霜霉病菌 Plasmopara viticola 具有抑制作用的 12 株细菌，其中抑制作用最强的为菌株 SY286，其在离体叶片上对葡 萄霜霉病防效可达 93.18%。在 2012—2013 年进行 2 次田间防效试验，结果表明菌株 SY286 发酵原液对葡 萄霜霉病防效可达 80% 以上。抑菌谱试验表明，该菌株对供试的 10 种植物病原真菌均有较好的抑菌作用， 抑菌带宽度为 5.93～24.90 mm。经形态观察、生理生化特征和 16S rDNA 同源性分析，鉴定为苍白杆菌 Ochrobactrum sp. 。 关 键 词：葡萄霜霉病菌；苍白杆菌；生物防治；叶际微生物 中图分类号：S476 文献标识码：A 文章编号：1005-9261 (2014)03-0402-06 Screening of Antagonistic Bacteria SY286 and Its Effect on Grapevine Downy Mildew 1, 2 2∗ 2 2 2 2 ZANG Chaoqun , ZHAO Kuihua , LIU Changyuan , LIANG Chunhao , YU Shuyi , LIU Li (1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; 2. Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China) Abstract ：Thirty-six samples of grapevine leaves were collected from nine locations in Liaoning province. Three hundred and three bacterial strains were isolated from leaves of grapevine for determining to antagonistic activity in vitro against Plasmopara viticola . Among the tested isolates, 12 isolates showed different levels of antagonism in detached leaf assay, strain SY286 reduced disease severity to the maximum level (93.18%) in detached leaves as well as showed good control effect (above 80%) in the field assay in 2012 and 2013. Inhibition spectrum tests showed that the strain SY286 could significantly inhibit against 10 tested fungal phytopathogens, and the