1与sE-selectin含量变化.docVIP

1与sE-selectin含量变化 第42卷第2期 2006年6月 青岛大学医学院 ACTAACADEMIAEMEDICINAEQINGDAOUNIVERSITATIS V01.42.No.2 June2006 急性脑梗死病人血清sICAM-1与sE-selectin含量变化 袁海成,赵仁亮 (1青岛市中心医院神经内科,山东青岛266O42;2青岛大学医学院附属医院神经内科) [摘要]目的探讨急性脑梗死病人血清可溶性细胞间黏附分子一1(slCAM1)和可溶性E_选择素(sE-selec— tin)水平的变化.方法采用双抗体夹心酶联免疫吸附法(EIASA)测定100例急性脑梗死病人发病第1,3,5,1O 天和3O例健康对照者的血清sICAM一1,sE-selectin的水平.结果急性脑梗死病人血清siCAM一1,sE—selectin的 浓度在发病第1天即升高.第3天达到高峰,第5天开始下降,第1O天显着下降.各时间点水平均高于对照组(F一 46.63,42.52,f一5.O7～18.07.Plt;O.O1);大梗死灶病人组血清sICAM1,sEselectin水平高于中梗死灶病人组和 小梗死灶病人组(F56.23,47.64,一4.74～9.26,Plt;0.01).结论急性脑梗死诱导血清steAM一1,sE—selectin 水平升高.随病程动态变化,且水平与脑梗死体积密切相关. [关键词]脑梗死}细胞间黏附分子;E选择素 [中图分类号]R743.3[文献标识码]A[文章编号]1672—4488(2006)02—0145—03 CHANGESOFSERUMslCAM一1ANDsE—selectinl—EVELSINPA1IENTSWITHACUTECEREBRALlNFARCTloNYLrAN HA—CHENG,ZHAOREN一,IANG(DepartmentofNeurology,QingdaoCentralHospital,Qingdao266042.China) [-ABSTRACT~ObjectiveToinvestigatethedynamicchangesofsolubleintercellularadhesionmolecule-1(slCAM1)and solubleE—selectin(sE—selectin)inacutecerebralinfarction.MethodsThelevelsofserumsiCAM一1andsE-selectininl00cases ofacutecerebralinfarctiononthe1st,3rd,5th,and10thdayaftertheonsetand30controlcasesweremeasuredseparatelyusing two—layerantibodysandwichenzyme—linkedimmunosorbentassay(ELISA).ResultsThelevelsofserumslCAM一1andsE—selec ninthepatientsincreasedsignificantlyonthe1stday,andreachedpeakuntilthe3rdday.followedbyobviousreductionafterfive and10days.ThelevelsofslCAM1andsE-selectinatalltime-pointincreasedsignificantlycomparedwiththecontrolcases(F= 46.63?42.52;t=5.07—18.07;Plt;0.01).LargercerebralinfarctioninducedhigherlevelofserumslCAM一1andsE—selectinthan themediumandsmalIcerebra¨nfarction(F=56.23.47.64;t=4.74—9.260Plt;0.01).ConclusionAcutecerebralinfarction increasesthelevelsofserumsiCAM-1andsE-selectin.whichischangedwiththecourseofthediseaseandcloselyassociatedwith volumeoftheinfaretedfocus. [-KEYWORDS~cerebralinfarction;intercellularadhesionmolecule;E—seleetin 脑梗死是中老年人常见病之一,有较高的致死 率及致残率.近年来脑梗死病人半暗区神经细胞损 伤与炎症的关系愈来愈受到人们的关注l1],脑缺血 受损部位有白