MS基本培养基成分调整对太行菊不定芽增殖的影响.docVIP

论文题目：MS基本培养基成分调整对太行菊不定芽增殖的影响 学生姓名： 所在院系： 所学专业： 导师姓名： 完成时间： MS基本培养基成分调整对太行菊不定芽增殖的影响 摘要 以太行菊不定芽块为实验材料，以MS为基本培养基，通过对比试验探讨了MS培养基中大量元素、Ca2+和K+、Fe2+不同浓度水平对太行菊不定芽增殖与生长的影响。结果表明：在MS基本培养基大量元素正常浓度水平下，太行菊不定芽块的增殖系数达到最高值8.3，大量元素浓度加倍时太行菊不定芽的增殖系数减小到7.1，但不定芽的长势稍强。在大量元素浓度正常和加倍情况下培养基中太行菊不定芽生长健壮，叶片舒展，叶色及芽点颜色浓绿，枯叶较少，长势较强。Ca2+和K+对太行菊不定芽的增殖与生长都有重要作用。Ca2+、K+同时加倍对太行菊不定芽增殖与生长有促进作用，不定芽的增殖系数为11.7，芽块比较大，但枯叶较多。K+单独加倍时，不定芽的增殖系数为11.4，芽块稍小，叶色浓绿，无枯叶，长势最好。与对照组相比，MS培养基中的Fe2+浓度加倍会使不定芽丛芽的增殖系数提高，太行菊不定芽叶色浓绿，芽点呈鲜绿色，但芽点细小，生长受到抑制；MS培养基中的铁盐浓度减半对太行菊不定芽的增殖与生长影响较小。综合来看，在Fe2+浓度正常的MS基本培养基上，太行菊不定芽增殖与生长状况较好。 关键词：太行菊，组织培养，不定芽，快速繁殖 The impact of MS basal medium composition adjustment on the Opisthopus taihangensis adventitious buds proliferation Abstract By adjusting the concentration of macroelement, Ca2+ and K+, Fe2+ in the MS medium, the proliferation and growth of Opisthopapus taihangensis adventitious buds were studied. The results showed that: Under the normal level of MS medium macro-elements, the proliferation coefficient of Opisthopapus taihangensis adventitious bud reached the highest value 8.3. Under the double level of macroelement the proliferation coefficient of Opisthopapus taihangensis adventitious buds decreased to 7.1, but the adventitious buds grew slightly robust. The element of Ca2+and K+ in the medium play an important role on the Opisthopapus taihangensis adventitious bud proliferation and growth. Under higher concentration of Ca2+, K+ in the proliferation medium, the proliferation coefficient of Opisthopapus taihangensis adventitious buds was 11.4, and the buds block was relatively larger compared with the control. When Fe2+concentration in the medium increased, the adventitious buds of Opisthopapus taihangensis grew normally, and the multiplication coefficient was lower (8.3). In addition, when the medium of Fe2+ concentration doubled, the Opisthopapus taihangensis adventitious shoots showed dark green, but the buds were smaller, its growth was inhibited. Compared wit