黄妍 Inhibition of PDCD6 Induces Cell Proliferation and.pptVIP

Results RNAi inhibited PDCD6 expression in ovarian cancer cells Inhibition of PDCD6 prohibited apoptosis and increased cell proliferation Inhibition of PDCD6 induced cell cycle 1. Inhibition of PDCD6 by RNAi induced cell proliferation and cell cycle in SKOV-3 and OVCAR-3 cells by up-regulating cyclin D1, cyclin B1, cyclin E, and proliferating cell nuclear antigen (PCNA). 2. Inhibition of PDCD6 by RNAi reduced apoptosis in SKOV-3 and OVCAR-3 cells by decreasing cleaved caspase-3, cleaved caspase-7, cleaved caspase-8, Bax, death receptor 5 (DR5）and death receptor 4(DR4) and increasing Bcl-2. * Inhibition of PDCD6 Induces Cell Proliferation and Reduces Apoptosis in Human Epithelial Ovarian Cancer Cells Yan Huang, Xiaohua Wu Department of Gynecology, Cancer Center, Fudan University Yan Huang Correspondence: Xiaohua Wu Ovarian epithelial cancer is the leading killer among all gynecological malignancies. Recent findings suggested that apoptosis and apoptosis-linked genes may play an important role in the development and progression of ovarian cancer (la Cour JM, et al. Mol Oncol. 2008). Programmed cell death gene 6 (PDCD6), also known as apoptosis-linked gene-2 (ALG-2), is a 22 kDa penta-EF-hand type Ca2+-binding protein that was first reported by Vito in 1996. It is well recognized as an apoptotic mediator because PDCD6 expression is required for apoptosis following numerous death stimuli (Vito P, et al. Science. 1996). Backgrounds Methods Poliferation BrdU Flow cytometry RT-PCR, Western blot Design of siRNA and construction of recombi-nant plasmids Flow cytometry Poliferation Apoptosis PDCD6 RT-PCR, Western blot RT-PCR, Western blot RT-PCR, Western blot BrdU, Flow cytometry Effects of cisplatin on ovarian cancer cells SKOV-3 and OVCAR-3 Transfection SKOV-3 and OVCAR-3 Cell cycle Apoptosis 18.57±1.53b 22.17±1.80b 59.27±0.90b OVCAR-3 pRT-pdcd 4.43±2.05 17.30±1.13 78.27±1.00 OVCAR-3 pRT-neg 2.93±1.08 17.17±1.11 79.90±1