超高效液相色谱法测定葡萄汁中单咖啡酰酒石酸的含量-食品科学.pdfVIP

※分析检测 食品科学 2013, Vol.34, No.10 135 超高效液相色谱法测定葡萄汁中 单咖啡酰酒石酸的含量 1,2 3 1 1,* 庞海霞 ，马鹏程 ，马 丽 ，张旺喜 (1.湖北工业大学化学与环境工程学院，湖北 武汉 430068；2.中国地质大学材料与化学学院，湖北 武汉 430074 ； 3.上海标检产品检测有限公司，上海 200 131) 摘  要：建立测定葡萄汁饮料中单咖啡酰酒石酸含量的超高效液相色谱法，对方法学进行了验证。结果表明在 0.50～50.00μg/mL范围内线性关系良好，方法的批内及批间准确度在97.1%～106.0%，精确度(RSD)小于4.1%，基 质加标回收率在10 1.8%～109.0%。用该法测定了市售葡萄汁饮料中咖啡酰酒石酸的含量，结果准确。 关键词：单咖啡酰酒石酸；葡萄汁饮料；超高效液相色谱；方法学验证 Determination of Cafteric Acid in Grape Juice Drink by Ultra-Performance Liquid Chromatography 1,2 3 1 1,* PANG Hai-xia ，MA Peng-cheng ，MA Li ，ZHANG Wang-xi (1. School of Chemical and Environment Engineering, Hubei University of Technology, Wuhan 430068, China ； 2. Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan 430074, China ； 3. STC (Shanghai) Co. Ltd., Shanghai 200 131, China) Abstract ：A method was proposed and validated for determination of cafteric acid in grape juice by ultra-performance liquid chromatography. The results showed that there was good linearity in the range of 0.50–50.00 μg/mL. The inter- batch and intra-batch precision (RSD) were between 97.1% and 106.0%, and the accurate (RSD) was less than 4.1%. The recoveries of cafteric acid from spiked samples ranged from 101.8% to 109.0%. The described method was successfully used for determination of cafteric acid in commercial grape juice drinks. Key words ：cafteric acid ；grape juice drink ；ultra-performance liquid chromatography ；method validation 中图分类号：O657.72 文献标志码：A