60 coγ辐射诱变小麦抗旱突变体筛选及分子标记检测 word格式.docxVIP

contentofsolublesugarandchlorophyllcontentandtotaldroughtresistant coefficientbehavedsignificantpositivecorrelation.Thecomplexdroughtresistancecoefficientwasusedtoidentifythedroughtresistanceoftheselines，accordingtothemean±2×standarddeviationofcomplexdroughtresistancecoefficient，InM3genertionofGUO9722，positivevariationoccurredin9lines，thepositivemutationratesfor3.96%，InM4genertion，positivevariationoccurredin14linesthepositivemutationratesfor6.16%.InM3 genertionofHeke2，positivevariationoccurredin9lines，thepositivemutationratesfor4.64%，InM4genertion，positivevariation occurredin10lines，thepositivemutationratesfor5.15%.Dependedoncomprehensiveperformancebetweentwogenerations，7droughtresistancemutantswasselectedinM4ofGUO9722group，5droughtresistancemutantswasselectedinM4ofHeke2group.ForGUO9722，ClusteranalysisshowedthatthegroupofM3canbedividedinto6groups whengeneticdistance was 1.59，the groupofM4canbedividedinto6groups whengeneticdistancewas1.96；ForHeke2，Clusteranalysis showedthat thegroupofM3canbedividedinto6groupswhengeneticdistancewas3.45，thegroupofM4canbedividedinto6groupswhengeneticdistancewas2.54.5.AccordingtoSSRidentificationindicated，therewere7and8linesofGuo9722andHeke2whosegeneticlocusmutated.InM4 progeniesofGuo9722，mutationoccurredingenome2A、3A、3B、3D、7D.Therewere8SSRlocuswhichmutated(26.7%)ingenomeA，7SSRlocuswhichmutated(23.3%)ingenomeB，15SSRlocuswhichmutated(50%)ingenomeD.InM4 progeniesofHeke2，mutationoccurredingenome2A、3B、3D、5D、7D.Therewere1SSRlocuswhichmutated(5.6%)ingenomeA，6SSRlocuswhichmutated(33.3%)ingenomeB，11SSRlocuswhichmutated(61.1%)ingenomeD.Keywords：Wheat?60Co-γray?Droughtresistancecoefficient；Droughtresistancemutant?SSR独创性声明本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，也不包含为获得安徽农业大学或其它教育机构的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了谢意。研究生签名：时间：年月日关于论文使用授权的说明本人完全了解安徽农业大学有关保留、使用学位论文的规定，即：学校有权保留送交论文的复印件和磁盘，允许论文被查阅和借阅，可以采用影印、缩印或扫描等复制手段保存、汇编学位论文。同意安徽农业大学可以用不同方式在不同媒体上发表、传播学位论文的全部或部分内容。(保密的学位论文在解密后应遵守此协议)研究生签名：时间：