探索性实验0612.ppt

DNA的固有免疫识别分子 要求 3，形式： 每个小组提交一份报告，字数3000左右，注明小组中每个同学的贡献。 幻灯片汇报及讨论 方法 查阅文献：综述和文献 应用现代免疫学的检测技术:如流式细胞术，ELISA技术、细胞免疫功能分析等 应用分子生物学的检测技术：如定量PCR， Insert pie diagram slide here. * Figure 1. PRRs Targeted by Vaccine Adjuvants TLR1, TLR2, TLR4, TLR5, and TLR6 are located on the cell surface. TLR2 forms a heterodimer with TLR1 or TLR6. TLR3, TLR7, and TLR9 translocate from the endoplasmic reticulum to endolysosomal compartments following cell stimulation. The CLRs are cell surface receptors, whereas the NLRs and RLRs reside in cytoplasm. The interaction of a PRR with its cognate ligand results in initiation of a signaling cascade leading to inflammatory responses. * * For this slide, begin with 3 BAC clones turning red and Tlr4 appearing. * New slide showing the pieces of evidence: photos of the original trace file; then the Northern; then the PCR; then the trace file. * Fig. 1. Putative RIG-I ligands. RIG-I has been reported to be triggered experimentally by a variety of RNA agonists. 5′-PPP–bearing RNAs are shown in green, RNAs without 5′-PPPs in blue, and RNAs that may have different 5′-end characteristics in orange. An antagonist is shown in black. Activated RIG-I promotes the induction of interferons and other pro-inflammatory cytokines via the mitochondrial adaptor MAVS (bold red arrows). MAVS-dependent induction of pro-interleukin-1b allows it to be processed into mature interleukin-1b by the inflammasome, which can be directly activated by RIG-I in a MAVS-independent manner. SCIENCE,2010;327:284 * * Fig. 1. Differential roles of retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA5) in RNA virus recognition. RIG-I recognizes 5￠-triphosphate RNA and short dsRNA, whereas MDA5 discriminates long dsRNA generated during the course of virus infection. RNA viruses are differentially recognized by RIG-I and MDA5. RIG-I is responsible for detecting Paramyxoviridae, Orthomyxoviridae, Rhabdoviridae, and some Flaviviridae family members. By