茶树上冰核活性细菌的分离鉴定及防治-安徽农业大学学报.PDF

安徽农业大学学报, 2011, 38(5): 788-791 Journal of Anhui Agricultural University [DOI]CNKI：34-1162/S1431.017 网络出版时间：2011-08-29 14:31:37 [URL]http:// /kcms/detail/34.1162.S1431.017.html 茶树上冰核活性细菌的分离、鉴定及防治 孙 健，何天良，江昌俊1* (安徽农业大学茶叶生物化学与生物技术教育部重点实验室，合肥 230036) 摘 要：霜冻发生时从茶树上分离到 1 株冰核活性细菌，该菌株在 ﹣5℃下在 2 min 内冻滴率达到85%，一个 冰核产生需要的细胞数约为 5.4×103 个，该菌株具有明显冰核活性。对该菌株进行 16S rDNA 序列对比分析，判断 该菌株为萎蔫短小杆菌（Curtobacterium flaccumfaciens ）。在 -5℃条件下，药剂防治效率顺序为：次氯酸钙＞Tween 80 ＞藏红＞亚甲蓝。在同一生态条件下的土壤中分离出 1 株拮抗菌，经鉴定该菌株为黄孢原毛平革菌(Phanerochaete chrysosporium) 。 关键词：冰核活性细菌；鉴定；药剂防除；拮抗菌 中图分类号：S571.1 文献标识码：A 文章编号：1672−352X (2011)05−0788−04 Identification and control of one strain of ice nucleation active (INA) bacteria isolated from the tea plant SUN Jian, HE Tian-liang, JIANG Chang-jun (Key laboratory of Tea Biochemistry and Biotechnology, Education of Ministry, Anhui Agricultural University, Hefei 230036) Abstract: One of ice nucleation active ( INA) bacteria was isolated from the tea plant when frost occurred ， and the freezing percentage of this strain was 85% at -5℃ in 2 min. The cells/ice nuclei were about 5.4×103, and the strain shows significant ice nucleation activity. After 16S rDNA sequences of the isolates in GenBank were analyzed, the strain was identified as Curtobacterium flaccumfaciens. The efficiency of chemical control order at ﹣5℃ was calcium hypochlorite Tween 80 saffron methylene blue. Under the same ecological condition, an- tagonist bacterium was isolated from the soil, which was identified as Phanerochaete chrysosporium. Key words: INA bacteria; identification; medicament control; antagonistic bacterium 茶树(Camillia sinensis)冻害的发生受多种因子 品采集范围为整个茶园，每种茶树采集 10 片老叶。 的影响。多数研究者认为，茶树低温冻害与低温及 对照菌株为大肠杆菌（E