3.0t mrdti及t2mapping成像在兔急性缺血性横纹肌溶解症中应用 word格式.docxVIP

中文摘要3.0TMRDTI及T2mapping成像在兔急性缺血性横纹肌溶解症中的应用小时开始出现进行性下降，缺血4小时下降至最低，缺血7小时ADC值开始出现缓慢升高趋势(P0.05)；FA值自缺血1小时开始出现进行性升高，缺血4小时达高峰，缺血7小时FA值开始出现缓慢下降趋势(P0.05)；T2值自缺血1小时开始出现进行性升高，缺血4小时达峰值，缺血6小时T2值开始出现缓慢下降趋势(P0.05)；HE染色见，D组缺血6小时出现颗粒变性，E组缺血8小时出现肌细胞溶解；ADC值、T2值改变与细胞凋亡指数改变呈中度相关，FA值与细胞凋亡指数改变呈低度相关；ADC值、FA值及T2值改变与活化的caspase-3蛋白的相对表达水平均呈中度相关。结论：1)ADC值、FA值和T2值能够及时、客观、准确量化反映缺血性横纹肌溶解症肌肉损伤从肌细胞水肿到肌细胞溶解的病理改变过程,纤维示踪成像能直观显示缺血肌肉肌纤维排列结构改变，DTI和T2mapping成像是评估横纹肌溶解症发生部位、程度及范围的较优无创检查方法。2)横纹肌溶解症急性期(8h)，肌肉损伤ADC值整体呈先下降后升高的趋势，FA值整体呈先升高后下降的趋势，T2值整体呈先升高后下降的趋势，纤维示踪可见肌纤维走行僵硬，部分肌纤维分离移位。3)T2WI-FS排除了脂肪组织干扰，是监测横纹肌溶解症损伤肌肉大体形态改变的较好序列。关键词：横纹肌溶解症，缺血，DTI，T2mapping，HE染色，细胞凋亡作者：邓美香指导老师：金泳海3.0TMRDTI及T2mapping成像在兔急性缺血性横纹肌溶解症中的应用英文摘要Theapplicationof3.0TMRDTIandT2mappinginthediagnosisofacuteischemicrhabdomyolysisofrabbitsAbstactObjective:Toobservethedynamicchangesandinvestigatethecorrespondingpathologicalbasisofmuscleinjuryofacuteischemicrhabdomyolysisofrabbitson3.0TMRDTIandT2mapping;toinvestigatethevalueof3.0TMRDTIandT2mappinginthediagnosisofacuteischemicrhabdomyolysis;toprovideevidencesforclinicalapplicationofMRDTIandT2mappingindiseaseevaluationofrhabdomyolysis.MaterialsandMethods:From30NewZealandwhiterabbits,2rabbitswererandomlychosentobecheckedbyDSAimmediatelyand8hourslateraftermodeling,provingthemodelingmethodsuccesful.Therestwererandomlyassignedinto5groups(n=6):groupA(normalcontrolgroup);groupB(ischemiagroupfor2hours);groupC(ischemiagroupfor4hours);groupD(ischemiagroupfor6hours);groupE(ischemiagroupfor8hours).GroupA,B,CandDunderwentMRI,histopathologicalexaminationanddetectionofapoptosisateachtimepoint.ButgroupEcontinuallyunderwentMRIbeforeischemiaand1~8hoursaftermodeling,9timesaltogether,then,underwenthistopathologicalexaminationanddetectionofapoptosisafterMRI.WecarriedoutMRIbyusingGESignaHDxt3.0Tsuperconductingmagnecticresonancescannerandsinglechannelflexsurfacecoil.WeconductedaxialT2WI-FS,T1WI,DTIandT2mapping.WeprocessedallthedataofDTIandT2mappinginGEADW4.4ImageProcessingWorkstation,measuringADC,FAandT2valueintherect