ghrelin对oxldl诱导内皮细胞损伤的保护及机制研究 word格式.docxVIP

①As shown in Hoechst 33258 staining assay, compared with the control group, thenumber of cells with nuclear condensation induced by ox-LDL (50mg/l) increased significantly. Pre-treatment with different concentrations (1, 10 or 100 ng/mL) of Ghrelin caused a decreased apoptotic proportion by 30%, 44%, 56%, respectively, compared with ox-LDL group (all p0.05). The apoptotics proportion of the HUVECs pretreated with 10ng/mL Ghrelin for different times (1, 6, 12 hours) was lowered by 40%, 60%, 72%, respectively, in comparison with ox-LDL-treated group (all p0.05).②Compared with the control group, expression of caspase-3 induced by ox-LDL(50mg/l) increased significantly. The expression levels with different concentrations (1, 10 or 100 ng/mL) of Ghrelin was decreased by 17%, 23%, 45%, respectively, and with10ng/mL Ghrelin for different times (1, 6, 12 hours) was lowered by 32%, 47%, 52%, respectively, between each group the differences were significant (p0.01 or 0.05).③As shown in nitrate reductase assay, compared with the control group, the NOproduction induced by ox-LDL (50mg/l) increased significantly (p0.01). Pre-treatment with different concentrations (1, 10 or 100 ng/mL) of Ghrelin caused an increased NO production by 25%, 54%, 85%, respectively, and 30%, 46%, 68% respectively for different times (1, 6, 12 hours), compared with ox-LDL group (p0.05).The inhibitory effect of Ghrelin on ox-LDL-mediated inflammatory cytokinesexpression in HUVECs①Compared with the control group, the expression levels of IL-6 in HUVECs treated with ox-LDL were significantly increased (p0.01), which was notably inhibited by19%, 35% and 60% decrease with different concentrations (1, 10 or 100 ng/mL) ofGhrelin, and by 36%, 45% and 56% decrease with different times (1, 6, 12 hours), between each group the differences were significant (p0.01 or 0.05).②Compared with the control group, the expression levels of IL-8 in ox-LDL groupwere significantly increased (p0.01). Pre-trea