表皮细胞生长因子复合骨髓间充质干细胞促进大鼠β射线皮肤损伤创面愈合的实验研究-烧伤外科学专业论文 word格式.docxVIP

表细胞生长因子复合骨髓间充质干细胞促进大鼠β射线皮肤损伤创面愈合的实验研究英文摘要Experimental Study on the Role of epidermal growth factor combined with Bone Marrow Mesenchymal Stem Cells in promoting β-ray irradiated Skin Injury Wound HealingAbstactObjectives: To investigate the effect and mechanism of the role of epidermal growth factor combined with bone marrow mesenchymal stem cells in promoting healing of β-ray irradiated skin inyury.Methods:1.3 SD rats(6-8 weeks old),clean grade, were killed using cervical dislocation method. We obtained the femur and tibia bone marrow under sterile conditions, isolated and cultured bone mesenchemal stem cells(BMSCs) by DiI when the passage grow into passage 3,and then we prepared the BMSCs suspension(1×106/ml in concentration).2.60 female SD rats (age:3-months old),clean grade. We applied single dosage(45Gy) of β-ray irradiation by linear accelerator on buttock skin (40mm×40mm) in rats, and established acute deep II β-irradiated skin injury model of rats. Those 60 SD female rats were randomly divided into 4 groups: EGF+BMSCs (Group A), EGF((Group B), BMSCs((Group C),normal saline control(Group D).In Group A,N=20,1ml BMSCssuspension(1×106/ml in concentration) were injected subcutaneously after the woundappeared, and EGF were sprayed to the wound regularly; In Group B, n=20, EGF was merely sprayed to the wound regularly, with the same method as the Group A; In Group C,n=20, BMSCs was merely injected when the wound appeared, with the same method as the Group A; In Group D, n=20,the control rats ,normal saline was injected when the wound appeared with the same method as the Group A. We observed the wound healing every week, and excised the wound tissue by 5mm×5mm in size under sterile conditions in the 1st,3,and 5th week after the treatment. The samples were examined with light microscopy, and immunohistochemistry stained with EGF,TGF,PDGF,NGF antibodies.Results:英文摘要 表细胞生长因子复合骨髓间充质干细胞促进大鼠β射线皮肤损伤创面愈合的实验研究Growth and observation of BMSCs: After 24 hours of