玻璃体腔注射鼠神经生长因子治疗视网膜色素变性的临床前分析-眼科学专业论文 word格式.docx

ONLOuter neuclear layer外核层PLRPupillary light reflex瞳孔光反射RCSRoyal College of Surgeons rats皇家学院外科大鼠RdRetinal degeneration视网膜变性RIARadioimmunoassay放射免疫分析RPRetinitis pigmentosa视网膜色素变性RPCRelative Pupillary Construction相对瞳孔收缩强度RPERetinal pigment epithelium视网膜色素上皮TGFβTransforming growth factorβ转化生长因子 βXLARX-linked recessive inheritance性连锁隐性遗传β-FGFβ-Fibroblast growth factorβ 成纤维生长因子Preclinical Study of Intravitreal Injection of Mouse Nerve Growth Factor for Retinitis PigmentosaAbstractRetinitis pigmentosa (RP) is a blinding disease characterized by progressive photoreceptors apoptosis. Studies demenstrated that the reduction of loss of several neurotrophic factors,such nerve growth(NGF), ciliary neurotrophic factor(CNTF), brain derived neurotrophic factor(BDNF), enhance the invasion and aggravation of RP. Thus one of the treatment strategy for RP is to make the neurotrophic factors of retina enough to the balance of retinal internal enviroment. Nerve growth factor(NGF) is one of the factors in neurotrophic factors family. The reduction or loss is correlated with the progress of RP. It indicated that NGF maybe one critical key factor implicated in RP. Eye local administration of exgenous NGF exerts a rescue effect on photoreceptors in the RP animal model. Furthermore, NGF not only protect photoreceptors itself, but also stimulate other biological mediators produced and released in the retina,such as brain-derived neurotrophic factor(BDNF), beta-fibroblast growth factor(b-FGF), transforming growth factor-beta(TGF), vascular endothelial factor (VEF)and neuropeptide-Y .The NGF ,a factor isolated from the mouse submandibular gland , has been the clinical i.m.medicine with CFA license，which gives the opportunity to the study of therapy ofretinal degeneration. However, the limitation of NGF useness in eye diseases is troublesome because of the little concentration contained in eye through NGF system administration. Intravitreal injection is a good technique of direct i