补肾益气方对家兔koa滑膜组织中aqp1和tnf-α表达的影响-中西医结合临床专业论文 word格式.docxVIP

Bushen Yiqi recipe on KOA in the synovial tissue of rabbit influence expression of AQP1 and TNF-alphAbstractObjection:Through the study of the kidney the profit gas traditional Chinese medicine through the research (FuFangJuanBi capsual) to experimental domestic rabbit knee osseous arthritis (KOA) in synovial membrane aquaporin AQP1 and expression of tumor necrosis factor TNF-α, to explore the mechanism of early prevention and treatment of BuZhongYiQi medicine of OA, to seek for OA early the effective treatment target point, for providing the reliable geography for the OA early Chinese medicine preventing and controlling to discuss the basis.Method:Selecting forty healthy Japanese long-eared rabbits were 4-month-old of male, the body weight is two point five to three point five. Dividing randomly for the blank group stochastically (A groups), physiological saline model group (B groups), hydrochloric acid glucosamine control group (C group) and BuZhongYiQi Chinese medicine treatment group (D group).The compatibility raises for one week later, starting to produce the mold. In addition to the blank group,other group animal uses the gypsum tubular-shaped to fix to the domestic rabbit knee osseous arthritis model makes the mold method, flanking the right knee joint will unbend outside the position gypsum the fixed 6 weeks, so that the right side of the knee can not be activities, to manufacture knee osseous arthritis model, after 6 weeks removes the gypsum.From makes the mold to start 1st day to start to gavage, the treatment course time is 4 weeks, the gavage case are as follows: A groups: not gavage; B groups: giveing the medication group constant volume (2 ml/d) the physiological saline fills the stomach every day, the minute will fill the clothing sooner or later twice; C group:every day to quite fill the stomach in the clinical adult dosage 5 times of glucosamines, will divide will fill the clothing sooner or later twice, the minute will fill the clothing sooner or