大黄灵仙颗粒剂对胆石症防治作用的基础与临床研究-中西医结合;临床专业论文 word格式.docxVIP

··················································27纳入标 准·························································································28排 除 标 准··························································································· 281.6疗效标 准····························································································291.7统计学标 准·······················································································29 1.8临床分 组···························································································291.9 药 物 使 用··························································································· 302．临床结 果··························································································· 303．临床讨论···························································································314．存在问题及展望 ·············································································33结论 ·······························································································34参考文献·································································································34 综述肝内胆管结石的病因学研究现状及治疗展望·················37 致谢······································································· ·························46攻读学位期间发表的学术论文题 目·····················································47 作者声明·································································································48 个 人 简 历·································································································49中文摘要目 的 ：大黄灵仙颗粒是在唐乾利教授“以防为主、从肝论治”的治疗 理念指导下的治疗胆石症有效方剂。本文旨在利用运用生物化学、分子 生物学实验手段从分子水平探讨胆石症的形成机制和中药的干预作用。 同时结合临床观察验证大黄灵仙颗粒的治疗作用，为中西医结合防治胆 石症提供理论及临床应用依据。方 法：1.实验研究：选健康新西兰大白兔共 60 只，分为模型组、治疗 组、正常对照组和药物对照组 4 组，每组 15 只。模型组和治疗组先进行 慢性肝损伤造模，完成后治疗组、药物对照组按剂量予大黄灵仙颗粒（制作成悬液）灌胃；模型组和正常对照组以等量生理盐水灌胃。5 周后取材， 观察、比较胆囊成石情况；检测血清生化指标；制作组织病理学、超微结构切片；运用分子生物学技术测定基因表达等方法进行大黄灵仙颗粒 干预作用机制的研究。2.临床研究：从广西中医学院第一附属医院、附属瑞康医院和广西自治区人民医院筛选胆石症病例 90 例，分为大黄灵仙组30 例（服用大黄灵