PPT整理 第11章Antigen开始.docVIP

第11章Antigen-Presenting Cells and Antigen Presentation What are APC and professional APC? APC :A variety of cell types which can take up, process antigen and carry antigen in a form (antigenic peptide-MHC molecule complex) that can stimulate lymphocytes. It is also known as accessory cell (A cell). professional APC: Dendritic cells, macrophages and B cells, which can express MHC class II molecules Describe MHC class I and MHC class II presentation pathways. ? MHC class I MHC class II Major antigen sources endogenous antigen exogenous antigen Processing machinery proteasome lysosomal enzymes, MIIC Cell type where active all nucleated cells professional APCs Site of antigen-MHC binding endoplasmic reticulum MIIC MHC utilized MHC class I MHC class II chaperone and transport molecule Calnexin, TAP Ii, Calnexin Presents to CD8+ T cell (Tc) CD4+ T cells (Th) Antigen presentation:The process by which certain cells in the body (APC) express antigen peptide-MHC molecule complex on their cell surface in a form recognizable by T lymphocytes. TAP is a heterodimer which uses ATP to help transport peptides (8-16 aa’s) to lumen of ER The difference between immature Dendritic Cell and mature DC. mature DC: Phenotype: low expression of PRRs (Toll-like recptors, mannose receptor), FcR and CR; high expression of MHC I/II and co-stimulatory molecule (CD54/ICAM-1 , CD40, CD80, CD86 ); CD1a and CD83+. Function: weak ability to capture and process Ags, powerful ability to present Ags. immature Dendritic Cell Phenotype: high expression of PRRs (Toll-like recptors, mannose receptor), FcR and CR;low expression of MHC II and co-stimulatory molecule Cellular organlle: MHC calssⅡcompartment, lysosome, Endosome Cytokine: Chemokine and proinflammatory CK such as IL-1, IL-6, IL-12, TNF-α secreted by LC Function: Powerful ability to capture and process Ags, but weak ability to stimulate T cells (or weak ability to present Ags); induction of immune tolerance What is Cros