孕妇外周血中胎儿DNA水平的检测用于诊断先兆早产.doc

孕妇外周血中胎儿DNA水平的检测用于诊断先兆早产 980Clinica1MedicalJournalofChina,2006.Vo1.13,No.6中国临库廛孝2006年12月第13卷第6期 ? 论着? 孕妇外周血中胎儿DNA水平的检测用于诊断先兆早产 王雪梅陶红纪向虹 摘要目的:探讨孕妇外周血中胎儿DNA水平的检测在先兆早产诊断中的应用价值.方法:选择5()例先兆早产孕妇,按 转归分为治疗无效的先兆早产组(30例),治疗成功的先兆早产组(20例),于有先兆早产征象时抽取外周血,另选5()倒正 常孕妇作为对照组(B超确定3组孕妇所妊娠的胎儿均为男性),对应治疗无效,治疗成功的先兆早产组的孕周时间抽取 外周血.标本采用荧光定量PCR检测胎儿Y染色体上的性别决定基因(SRY基因)DNA水平,使用阴道超声诊断仪测量 宫颈长度.结果:(1)治疗无效的先兆早产组胎儿DNA水平为(625.5±52.4)copy/ml;治疗成功的先兆早产组胎儿DNA 水平为(220.4±57.5)copy/m[;对照组胎儿DNA水平为(189.2±43,2)copy/m1,治疗成功的先兆早产组较对照组明显升 高,治疗无效的先兆早产组较治疗成功的先兆早产组明显升高,两者均有显着差异(Plt;().(1).(2)治疗无效的先兆早产 组平均宫颈长度为(28.9±6.8)mm,治疗成功的先兆早产组平均宫颈长度为(41.8±6.4mm),对照组平均宫颈长度为 (42.2±5.8)mm.治疗无效的先兆早产组与治疗成功的先兆早产组比较,Plt;0.01;治疗无效的先兆早产组与对照组比较 有显着差异(Plt;0.01);对照组与治疗成功的先兆早产组比较无显着差异(Pgt;0.05).结论:孕妇外周血胎儿DNA水平 变化可作为预测先兆早产一个有应用价值的指标. 关键词DNA;先兆早产;聚合酶链反应;官颈长度 中图分类号R714.21文献标识码A RoleofMeasuringtheConcentrationofCirculatingNon-hostfetalDNAofPregnantWomenPlasnminPrenm- tureDeliveryWANGXuemeiTAOHongJIXianghongDepartmentofObstetrics,QingdaoMunici 加ZHospitalinShandongProvince,Qingdao266071 AbstractObjective:ToinvestigatetherelationshipbetweentheconcentrationoffetalDNAinpregnantwomenplasmaand prematuredelivery.Methods:50casesconsistingof30prematuredeliverywomenand20threatenedprematuredeliveryandan— other5OcasesofnormalpregnancywereselectedtoqualifySRYlevelsinmaternalbloodwithafluorescentquantitativepoly— merasechainreaction(QF_PCR)andmeasuretheconcentrationoffetalDNAwithSRYgene.Thegestationalagesofthetwo groupshavenosignificantdifferenceandallfetusweremaledetectedbyBultrasonic.thecervixlengthwasmeasuredwithtrans— vaginalsonography.Results:(1)thefetalDNAconcentrationof30prematuredeliverycasesis625.54±52.4copy/ml:while theother20threatenedprematuredeliverycasesis220.4±57.5copy/m1.thecontrolgroupis189.244-43.2copy/rn1.Theva— fianceanalysisandimpairQtestindicatethatthefeta1DNA1evelinmatema1bloodoftheprematuredeliveryishigherthanthe threatenedprematuredelivery,anditisalsohigherinthreatenedprematuredeliverygroupthaninthenormalgroup.Boththed ifferencesares