深度测序技术简介推荐.pptVIP

Digital expression profiling microRNA re-sequencing： hESC： human embryonic stem cells EB： embryoid bodies 绿宇生物科技 专业建库 测序 QQ:110199525 ChIP-seq（1）： 人一号染色体DNA-蛋白相互作用 绿宇生物科技 专业建库 测序 QQ:110199525 ChIP-seq（2）： Sequenced short reads (typically
25–50 bp) from ChIP-Seq experiments are ?rst mapped onto the reference genome. The mapped reads are then used to estimate statistical parameters, which include the estimation of the average length F of sequenced DNA fragments. 绿宇生物科技 专业建库 测序 QQ:110199525 Methy-seq（1）： 肿瘤和MCF7细胞系中 BRCA!启动子区域的甲基化差异 绿宇生物科技 专业建库 测序 QQ:110199525 Some highlights:Correlation between ChIP-Seq and his prior SAGE-like method (called GMAT) has r=0.906‘However the resolution with ChIP-Seq was dramatically higher. Furthermore, ChIP-Seq was more sensitive and generated less false-negative regions’12,726 genes whose transcription levels are known in CD4+ T-cells were correlated with the histone modifications and 35,961 Pol II binding site ‘islands’ were identified‘This cost-effective method produces digital-quality data and should find broad applications in our efforts to understand the contribution of the human epigenomes in gene expression and epigenetic inheritance’ Methy-seq（2）： 绿宇生物科技 专业建库 测序 QQ:110199525 Genome re-sequencing van Orsouw N J, Hogers R C, Janssen A, et al. Complexity reduction of polymorphic sequences (CRoPS): a novel approach for large-scale polymorphism discovery in complex genomes. PLoS ONE, 2007, 2(11): e1172 Hillier L W, Marth G T, Quinlan A R, et al. Whole-genome sequencing and variant discovery in C. elegans. Nat Methods, 2008, 5(2): 183—188 Transcriptome re-sequencing Mortazavi A, Williams B A, McCue K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods, 2008, 5(7): 621—628 Sugarbaker D J, Richards W G, Gordon G J, et al. Transcriptome sequencing of malignant pleural mesothelioma tumors. Proc Natl Acad Sci USA, 2008, 105(9): 3521—3526 Digital expression profiling Ruby J G, Jan C,