仿刺参卵多糖的分离纯化及体外抗肿瘤活性-食品科学.pdfVIP

※基础研究 食品科学 2016, Vol.37, No.23 105 仿刺参卵多糖的分离纯化及体外抗肿瘤活性 1 1 1,2,* 3 1 1 1 刘 昕，刘京熙，张 健 ，王 婷 ，王共明，井月欣，赵云苹 （1.山东省海洋资源与环境研究院，山东 烟台 264006 ；2. 山东省海洋生态修复重点实验室，山东 烟台 264006 ； 3.上海海洋大学食品学院，上海 201306 ） 摘  要：目的：探究仿刺参卵多糖的结构及体外抗肿瘤活性。方法：通过酶解醇沉获得仿刺参卵粗多糖 polysaccharides from Apostichopus japonicus spawn SPS （ ， ）；通过色谱柱纯化后进行理化性质分析；采用噻唑蓝 法研究卵多糖对HeLa 、HGC-27细胞的体外增殖抑制作用。结果：获得3 种不同多糖组分SPS-1、SPS-2、SPS-3， 对干预的肿瘤细胞均有显著抑制作用。SPS-1抑制作用最强，对HeLa及HGC-27细胞的最高抑制率分别为98.04%、 99.4%。结论：从仿刺参卵中分离出3 种多糖组分，均表现出一定的抑制肿瘤细胞增殖活性。 关键词：仿刺参卵；多糖；纯化；抗肿瘤活性 Purification and Antitumor Activity in vitro of Polysaccharides from Apostichopus japonicus Spawn 1 1 1,2,* 3 1 1 1 LIU Xin , LIU Jingxi , ZHANG Jian , WANG Ting , WANG Gongming , JING Yuexin , ZHAO Yunping (1. Shandong Marine Resource and Environment Research Institute, Yantai 264006, China; 2. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China; 3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China) Abstract: Purpose: To investigate the structural characteristics and antitumor activity in vitro of polysaccharides from Apostichopus japonicus spawn. Methods: The polysaccharides from Apostichopus japonicus spawn (SPS) were isolated by protease hydrolysis and alcohol precipitation, and then purified by chromatography. Their physicochemical properties were analyzed. Their inhibitory effect on the growth of HeLa and HGC-27 cells was evaluated using methyl thiazolyl tetrazolium assay. Re