第十三章 双水相萃取 生物制药工艺学.pptVIP

第十三章 双水相萃取 第十三章 双水相萃取（aqueous two-phase extraction） 潘薛波 手机号码：649951 E-mail：xuvgou@ QQUpstream Processes Mid-processes fermentation downstream section Purification The major process bottleneck has been found in the selective purification steps, currently dominated by packed-bed chromatography. packed-bed chromatography it has also been the major cost centre mainly due to media cost and relatively long cycle times. higher-titre processes have been imposing practical limitations that will make the current technology platforms reach their limits of throughput and scalability. large columns also suffer from scale-related packing problems, such as hysteresis, edge-effects and resin compression which may result in unpredictable fluid distribution and pressure drops. Several alternatives to the established platforms have generated long-standing interest either to replace column chromatography or to eliminate the number of chromatography steps by reducing the load of impurities in the feed stream Examples are flocculation, precipitation, crystallisation, high gradient magnetic fishing, membrane chromatography, filtration and liquid–liquid extraction Conventional liquid–liquid extraction, using organic-aqueous phase systems, is a classical and versatile technology that has been established as a workhorse in the pharmaceutical industry. poor solubility and possible denaturation of the proteins in organic solvents. limited to the recovery of low molecular weight products, such as antibiotics and organic acids from fermentations broths. Liquid–liquid extraction based on aqueous two-phase systems (ATPSs) has shown a great potential and versatility for the downstream processing of biopharmaceuticals, such as monoclonal antibodies, high density lipoproteins, hormones, cytokines, growth factors and plasmid DNA. 早在1896年,Beijerinck发现,当明胶与琼脂或明胶与可溶性淀粉溶液相混时,得到一个混浊不透明的溶液，随之分为两相,上相富含明胶,下相富含琼脂(或淀粉), 这种现象被称为聚合物的不相溶性（incompatibility），从而产生了双水相体系(Aqueous two phase system,ATPS)。