苦豆籽粕-双歧杆菌合生元对aa+肉仔鸡肠道微生态系统影响的研究-临床兽医学专业论文.docx

Studyonthe Effects oftheFoxtail-likeSopharaSeed’sDregs-Bifidobacterium Synbiotics onMicroecosystemofIntestinal TractInternalEnvironmentinAA+broilersAbstractInordertoexploreanew,safeandgreentypemicroecologypreparationforforage,the Foxtail-likeSopharaSeed’sDregsandBifidobacteriumbifidumweretakenasmain experimentalmaterialinthisresearch.ThisexperimentstudiedtheeffectoftheFoxtail-like SopharaSeed’sDregs-Bifidobacteriumsynbioticsonperformaceandintestinaltract internalenvironmentinbroilers.Inthisexperiment,weaddedthemicroencapsulatedof Foxtail-likeSopharaSeed’sDregsandBifidobacteriumbifidumtobroilerfeed.ThenstudyontheeffectofthenumberofBifidobacterium、LactobacillusandE.coli.Toprovideatheoreticalbasisforthefurtherdevelopmentofnew,greenprobiotics.420onedayoldarberacresbroilerswererandomlydividedinto6groups.GroupⅠwasthecontrolgroup,whichfedbasaldiet(noantibiotics).GroupⅡwasfedbasaldiet+0.02% Bifidobacteriumbifidumfreeze-driedpowder.GroupⅢwasfedbasaldiet+2% Foxtail-likeSopharaSeed’s Dregs.GroupⅣ、Ⅴ、Ⅵwas fedbasaldiet，towhich0.5%、 1%、2%Synbioticswereaddedrespectively,forthe42daysexperimentalperiod.Throughalltheexperimentwithoutantibioticscompletely.10broilersineachgroupwere randomly selectedin7d,14d,21d,28d,35d,42dtodeterminethenumberofBifidobacterium、LactobacillusandE.coliinleftAppendixusedQuantitativePCR.Wechoose BifidobacteriumandLactobacillusonSelectivemediumanddothestrainidentification. The results areasfollows：Onageof7th，Thebifidobacteria’snumberofallthesynbioticsishigherthan control; Theresultsshowedthat:Foxtail-likeSopharaSeedcansignificantlyinhibitthegrowthof E.coli.0.5%and1%synbiotics cansignificantlyincreasedthenumberofE.coli.Onageof 14th,2%synbioticscan significantlyincreasedthenumber of bifidobacteria.Onageof21th,GroupofBifidobacterium、1%synbioticsand2%synbioticscansignificantlyincreasedthenumberofbifidobacteria,1%synbioticscansignificantly increasedthenumberofLactobacillus.2%synbioticscansignificantlyinhibitthegrowth ofE.coli.Onageof28th,Foxtail-likeSopharaSeedcans