脂肪干细胞的分化相关.pdf

Tratwal et al. Stem Cell Research Therapy (2015) 6:62 DOI 10.1186/s13287-015-0062-9 RESEARCH Open Access Influence of vascular endothelial growth factor stimulation and serum deprivation on gene activation patterns of human adipose tissue- derived stromal cells * Josefine Tratwal, Anders Bruun Mathiasen, Morten Juhl, Sonja Kim Brorsen, Jens Kastrup and Annette Ekblond Abstract Introduction: Stimulation of mesenchymal stromal cells and adipose tissue-derived stromal cells (ASCs) with vascular endothelial growth factor (VEGF) has been used in multiple animal studies and clinical trials for regenerative purposes. VEGF stimulation is believed to promote angiogenesis and VEGF stimulation is usually performed under serum deprivation. Potential regenerative molecular mechanisms are numerous and the role of contributing factors is uncertain. The aim of the current study was to investigate the effect of in vitro serum deprivation and VEGF stimulation on gene expression patterns of ASCs. Methods: Gene expressions of ASCs cultured in complete medium, ASCs cultured in serum-deprived medium and ASCs stimulated with VEGF in serum-deprived medium were compared. ASC characteristics according to criteria set by the International Society of Cellular Therapy were confirmed by flow cytometry. Microarray gene expressions were obtained using the Affymetrix HT HG-U133+ GeneChip®. Gene set enrichment analysis was performed using the Kyoto Encyclopedia of Genes and Genomes and gene ontology terms. Transcription of selected genes of interest was confirmed by quantitative PCR. Results: Compared to ASCs in complete medium, 190 and 108 genes were significantly altered by serum deprivation and