专业英语-天然药化.pptVIP

English for Natural Products Chemistry 1. Isolation and Chromatography 1.1 Paper Chromatography 1.2 Thin Layer Chromatography 1.3 High Performance Liquid Chromatography In search for bioactive constituents, we carried out chemical studies on Panax ginseng. We have systematically investigated the chemical constituents of Panax ginseng. detector：检测器 UVD: ultraviolet-visible detector (紫外可见吸收检测器) PDAD: photodiode array detector (光电二极管阵列检测器) RID: refractive index detector (示差折光检测器） FD: fluorescence detector (荧光检测器) ECD: electrochemical detector (电化学检测器) ELSD: evaporative light-scattering detector (蒸发光散射检测器） HPLC is analogous to GLC in its sensitivity and ability to provide both quantitative and qualitative data in the single operation. It differs in that the stationary phase, bonded to a porous polymer is held in a narrow-bore stainless steel column and the liquid mobile phase is forced through under considerable pressure. The apparatus for HPLC is more expensive than GLC, mainly because a suitable pumping system is required and all connections have to be screw-jointed to withstand the pressures involved. The mobile phase is a miscible solvent mixture, which either remains constant (isocratic separation) or, may be changed continuously in its proportions, by including a mixing chamber into the set-up (gradient elution). The compounds are monitored as they elute off the column by means of a detector, usually measuring in the ultraviolet. A computing integrator may be added to handle the data as they emerge and the whole operation can be controlled through a microprocessor. 2. Spectral Identification2. 1 ULTRAVIOLET-VISIBLE (UV-VIS) SPECTROSCOPY紫外-可见（UV-VIS）吸收光谱 electromagnetic spectrum 电磁谱 infrared：红外 visible light：可见光 wave numbers: 波数 proportional to energy: 能量成正比 red light: 红外线 violet light: 紫射线