bmp 2活性肽纳米晶胶原基骨修复材料复合移植修复大鼠颅骨缺损的实验研究word格式论文.docxVIP

【结论】①P24/ nHAC 复 合 材 料促进骨缺损修复能力明显强于空白 的nHAC， 略 低 于 3ug 剂量的 rhBMP2/ nHAC 复 合 材 料 。 ② nHAC 是 一 种 理 想 的生物支架和缓释材料。③ P24/ nHAC 复 合 材 料 是一种理想的具有稳 定骨诱导活性的新型骨缺损修复材料。 【关键词】BMP-2 活性多肽；颅骨缺损；纳米晶胶原基支架；骨诱导 ；骨组织 工程。 Experimental Study of Repair of Cranial Bone Defects using BMP-2 derived bioactive peptide combined withnano-hydroxyapatite and Collagen IAbstractObjective: To investigate the osteogenetic capacity of bone repair in rat cranial bone defects by nHAC loading with a BMP-2-derived peptide P24 biomimetic scaffold materials.Methods:19 male Sprague–Dawley rats were divided into four groups. Five-millimeter critical-size cranial bone defects were created in each one. The defects were treated with P24/nHAC scaffold (A group), rhBMP-2/ nHAC scaffold (Bthgroup), nHAC scaffold (C group)and only bone defect(D group). Up to the 6thand 12weeks, the rats were sacrificed in batch respectively. Defects were evaluated by X-ray、three-dimensional reconctruction of computer tomography 、histology and the percentage of bone formation areas.Results: ①Radiological examination indicated that there were some flakyradiodense areas in A and B gruoups of each rat, but the density of the radiodensethareas of C group were obviously lower than the density of A and B groups at the 6thweek.At the 12 week,the radiodense areas of C group were denser than the 6thweek,but defects not repaired. The defects nearly healed at the 12week in the A andB groups. A trifle of radiodense areas were discovered in the margin of defects in the D group.②Three-dimensional reconstruction of computed tomography indicated that there were fewer radiodense areas and not saw bone bridge in the C group, but thedensity of the radiodense areas of A and B groups were obviously denser than thethdensity of C group and bone brige was discovered at the 6week. The defectscompletely healed in B group and nearly reparied in A group that was treated withthBMP-2- derived peptide loaded nHAC at the 12week ,whi