cox2 p53在前列腺癌组织中表达及其临床意义word格式论文.docxVIP

得数据应用SPSS13.0 统计软件包进行进行统计学分析。结果：免疫组化结果显示COX-2、p53在前列腺癌组织、前列腺增生组织、正常前列腺组织表达强度依次下降，且与前列腺癌患者Gleason 评分、肿瘤分期、淋巴结转移、3 年、5 年生存率相关。在前列腺癌组织中COX-2mRNA、p53mRNA 表达水平明显高于前列腺癌瘤旁无瘤组织通过RT-PCR得到验证，进一步验证在前列腺癌组织细胞中COX-2 mRNA 、p53mRNA呈特异性表达。结论：通过本课题研究，探求COX-2、p53与前列腺癌的相关性，证实COX-2 、p53 与前列腺癌的发生、进展、侵袭、预后密切相关，对前列腺癌患者具有早期诊断、预防、治疗以及判定预后的价值，有望成为一种具有临床实用意义肿瘤标志物。COX-2 、p53 两种蛋白在PCa 组织中的表达存在正性相关，二者联合检测可为前列腺癌发生发展及预后预测提供一定的参考价值。关键词：前列腺癌、COX-2、p53、免疫组化、RT-PCRTheexpressionsofCOX-2 andp53 in Prostate Carcinomaandits clinicalsignificancePostgraduate:YaoWen (Urology) Tutor:professorHuJian-BoAbstractBackground:Prostatecancer isahighincidence in oldermen。ProstatecancerinEuropeand theUnitedStatesaccountedforthefirstofthemalignanttumorsinthemalereproductive system,themortalityraterankedsecond,secondonlytolungcancer.RecentincidenceofProstatecancerinChinaisincreasingrapidly.Menarecurrentlydiagnosedatan earlystageInmany cases,prostatecancercanbe surgeryor radiationtherapy.However, theone-thirdofpatients,thediseasewillrelapse,andultimatelydevelopintoHormone refractoryprostatecancerandmetastaticprostatecancer.Asanewtreatmentforcancer genetherapyis considered to beincreasing.ItisreportedthatCOX-2 theheightoftheepitheliuminavarietyof regulatoryMalignanttumorssuchaslung,stomach,colonCervicalcancerandovarian cancer. COX-2 hasbeenreportedTumorstage,tumorgrade,histologicalgradeand prognosis.Therefore,COX-2may beapotentialmoleculartargetofcancertherapy Treatment.P53 isanimportanttumorsuppressorgenetopreventcellreplicationandThe maintenanceofgenomestability,maintainingnormal cell growth and promote apoptosisInhibittheproliferationofmalignantcells,thegrowthregulatorisanegative impact.Its Mutationandinactivationplaysanimportantroleintheprocessoftumor growthand Infiltration. Thisindicatesthatp53 genemutationmaybecloselyrelatedto aAvarietyofhumantumors.Lungcancer,coloncancer,breastcancer,livercancer, prostate cancer Cancer, bladder cancer, ovarian cancer,brain tumor, nasopharyngealcarcinoma,Thep53geneishighlyexpressedi