dj1对卵巢癌skov3细胞增殖 凋亡和侵袭影响word格式论文.docxVIP

ABSTRACTObjective:TheaimofthisstudywastoexploretheeffectofDJ-1ontheproliferation，apoptosisandinvasionofovariancancerSKOV3cellsafterDJ-1silencingbyRNA interference.Methods:DesignandsynthesisthreepairsofspecificsiRNAtargetingDJ-1,and transientlytransfectedintoSKOV3cellsvialiposometransfectionmethod. Thestudy weredividedintofive groups：normal controlgroup,negativecontrol group，siRNA-DJ-1-agroup，siRNA-DJ-1-bgroup，siRNA-DJ-1-cgroup.WesternblottingwasusedtodetecttheexpressionofDJ-1andSelectingthebestinterferencesequence.Theabilitiesofproliferation，apoptosisandinvasionofDJ-1wereassessedbyMTTassay，Flowcytometryandtranswellassay,respectively.Results:WesternblottingwasusedtodetecttheDJ-1proteinexpressionafter transfectionofsiRNA.TheresultsshowedthatthelevelofDJ-1protein inthesiRNA-DJ-1groupsweresignificantlylowerthannormalcontrolgroupandnegativecontrolgroup（0.07±0.01、0.11±0.02、0.10±0.01vs0.51±0.02、0.50±0.02，P0.05）,whiletherelative DJ-1protein expressionlevelsofnormal controlgroupandnegativecontrolgroup showed nosignificantdifference（0.51±0.02vs0.50±0.02，P0.05）.TherewasasignificantdifferenceofsiRNA-DJ-1-agroupcomparedto siRNA-DJ-1-bgroupand siRNA-DJ-1-c group（0.07±0.01vs0.11±0.02，0.07±0.01vs 0.10±0.01，P0.05）.MTTassaywasusedtodetectcellproliferation.24h,48hand72hafter transfection,theresultsshowedthatsiRNA-DJ-1-agroupcellproliferationratewas lowerthannormal control group(24h，0.379±0.044vs0.492±0.035；48h，0.486±0.059vs1.127±0.078；72h，0.738±0.046vs1.373±0.055,P0.05)andnegativecontrolgroup(24h，0.379±0.044vs0.460±0.057；48h，0.486±0.059vs1.043±0.080；72h，0.738±0.046vs 1.350±0.067,P0.05).Flowcytometrywasusedtodetectcellapoptosis.TheresultsshowedthattheapoptosisrateofsiRNA-DJ-1-agroupwassignificantlyhigherthannormalcontrolgroup（27.97±9.86% vs 1.77±0.71%, P0.05）and negative control group（27.97±9.86%vs2.83±0.55%, P0.05）, whiletheapoptosis rateofnormal controlgroupandnegativecontrolgroupshowednosignificantdifference(1.77±0.71%vs2.83±0.55%，P0.05).transwellassaywasusedtodetectcellinvasion.Th