exendin4抑制tbhp诱导huvecs凋亡与pi3kaktbcl2通路有关word格式论文.docxVIP

nmol/l),cellviabilityincreasedby46.5%(P0.01),andthenumberofapoptosisdecreasedsignificantly(8.0%±1.45vs36.0%±1.25,P0.01),comparedwiththet-BHPtreatedgroupalone.Goingafurtherstep,weexaminedtheunderlyingregulation.Pre-incubationofHUVECswithexendin-4resultedareductionofactivecaspase-3(by48.53%,P0.01),anenhancementofphosphorylatedAkt(by1.78 times,P0.01)andanincreaseinanti-apoptoticproteinBcl-2expression(by1.08 times,P0.01),comparedwiththet-BHPgroup.Theseresultsindicatethatexendin-4canprotectt-BHP-inducedHUVECsapoptosis.However,thisprotectiveeffectwereinhibitedbythephosphatidylinositol-3kinase(PI3k)inhibitorwortmannin.Conclusion:Ourfindingsshowedthatoxidativestressinducescaspase-dependentHUVECapoptosis,whichispartlymediatedbytheinactivationofAktandthereductionofBcl-2expression.GLP-1analogueexendin-4hasaprotectiveeffectagainstoxidativestress-inducedendothelialcellapoptosis,whichmayassociatetotheinvolvementofsurvivalpathwayPI3k/Akt-Bcl-2.Keyword:oxidativestress;Humanumbilicalveinendothelialcells;Exendin-4;PI3k;Akt;Bcl-2缩略词表英文缩写英文全称中文全称ASAtherosclerosis动脉粥样硬化t-BHPtert-Butyl hydroperoxide第三丁基过氧化氢GLP-1Glucagon-like peptide-1胰高血糖素样肽-1 HUVECsHuman umbilical vein endothelial cells人脐静脉内皮细胞 PIPropidium Iodide碘化丙啶FBSFetal bovine serum胎牛血清 DMEMDulbecco’smodifiedEagel’smediumDMEM培养基 PBSphosphate buffered saline磷酸盐缓冲液DMSOdimethyl sulfoxide二甲基亚砜APSAmmonium Persulfate过硫酸铵SDSSodium Dodecyl Sulfonate十二烷基硫酸钠 PI3Kphosphatidylinositol 3 kinase磷脂酰肌醇3激酶 Akt/PKBprotein kinase B蛋白激酶BROSreactive oxygen species活性氧族PKCprotein kinase C蛋白激酶CPKAprotein kinase A蛋白激酶AAGEsadvanced glycation end-products晚期糖基化终产物 cAMPcyclic adenosine monophosphate环腺苷一磷酸 DDP-IVdipeptidyl peptldase Ⅳ二肽基肽酶Ⅳox-LDLoxidative low density lipoprotein氧化型低密度脂蛋白MMPmatrix metalloproteinase基质金属蛋白酶NOnitric oxide一氧化氮eNOSendothelial nitric oxide synthase内皮型一氧化氮合酶学位论文原创性声明和版权使用授权书学位论文原创性声明本人郑重声明：所呈交的学位论文，是本人在导师的指导下，独立进行研究工作所取得的真实成果。除文中已注明引用的内容外，本论文不含任何其他个人或集体已经发表或撰写过的作品成果。对本论文的研究做出重要贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。作者签名（手写）：导师签名（手写