fbxw7 p53与aurora审审a在胃癌组织中的表达和临床意义word格式论文.docx

Expressionof FBXW7,Aurora-AandP53GeneintheGastric Carcinomaandits ClinicalSignificanceAbstract:Objective:ToinvestigatetheexpressionofFBXW7,Aurora-AandP53genesingastriccarcinomaanditscorrelationwithclinicopathologicfeaturesofgastriccarcinomaandtoexploretheroleofthedevelopmentofthevariousindicatorsingastriccancer.Methods:TheFBXW7,Aurora-AandP53gene were detected by HE staining and immunohistochemical S-Pmethods in gastric carcinoma tissue and normal gastric tissue,observetheirindividualandjointexpressionofthesituation,andtherelationofexpressionofFBXW7,Aurora-AandP53geneswiththedevelopment,progression,metastasisandprognosisofgastriccarcinomatissue.1.Thesurgicalresectionandparaffinblocksofgastriccancer confirmedby pathologyspecimensof60casesand20casesofnormalgastricmucosaarchiveparaffinblockswereobtainedfromtheDepartmentofPathologyoftheThirdPeople’sHospital ofMianyang City, September 2007 to September 2011. 2.Theinclusion criteria: ①All the patients were not given chemotherapy andradiotherapybeforepreoperation,andallthepathologicalspecimenswereconfirmedasgastriccancerafterpostoperation.②Allthespecimenswerefixedby 10﹪formalin, embedded in paraffin, 5μm serial sections.③20 cases ofnormalgastricmucosawerealltakenfrom anormalgastroscopyspecimens.The exclusion criteria:①All the patients were given chemotherapy andradiotherapybeforepreoperation,andallthepathologicalspecimenswereconfirmasgastriccancerafterpostoperation.②Allthenormalgastricmucosawereconfirmedgastriccancerorsuspectedgastriccancerbypathology.3.UsingimmunohistochemicalSPmethod,DABcolor.Anti-FBXW7,P53andAurora-AmonoclonalantibodytocomparepathologicalobservationrecordsFBXW7,P53andAurora-Aexpressioningastriccancerandnormalgastricmucosatissuesamples.4.Thestatisticaldate was analyzed bystatisticalsofeware 13.0SPSS,FBXW7,P53and Aurora-Aexpressioningastriccancer with χ2 test,p＜0.05wasconsideredtobesignificant.Results:1.TheexpressionofFBXW7,Aurora-A and P53 genes in gastric c