ets1和vegf在肺腺癌组织中的表达及意义word格式论文.docx

which play an important role in altering the extracellular matrix and angiogenesis, such as matrix metalloproteinases(MMPs), urokinase plasminogen activator（uPA）and so on, participating extracellular matrix degradation, cell migration,tumor angiogenesis, invasion and metastasis and many other areas. It is reported in the literature that Ets-1 and vascular endothelial growth factor (VEGF) have an intimate relationship in the process of infiltration and metastasis as well as angiogenesis in noumenal tumor.ObjectiveTo detect Ets-1 and VEGF protein expression level in lung adenocarcinoma tissue and far-adjacent normal lung tissue, analyze their dependablity with clinical pathological data such as degree of tumorcell differentiation, lymph node metastasis, clinical stage, and study the correlation of Ets-1 and VEGF to the occurrence, development and clinical features of lung adenocarcinoma.MethodsIn this study, immunohistochemical staining (SP method) was used to detect the protein expression of Ets-1 and VEGF in 46 cases of lung adenocarcinoma and 16 cases of adjacent normal tissue (both were archived wax blocks of surgical removal tissue). We also analyze the correlation between them, and explore their clinical significance. All specimens were pathologically confirmed lung cancer tissue or normal lung tissue.ResultsEts-1 and VEGF had different levels of expression in 46 cases of lung adenocarcinoma tissue: Ets-1 positive expression rate was 71.74% (33/46), VEGF positive rate was 76.09% (35/46). Both were obviously higher than them in16 cases of adjacent normal tissue:,12.50% (2 / 16) and 25.00% (4 / 16), the difference had statistically significant (P 0.05). In lung adenocarcinoma tissues, following with the average diameter increasing, degradedness of tumor differentiation degree and lymph node metastasis, the positive expression of Ets-1was increased, and the difference was statistically significant (P 0.05); The positive expression of VEGF closely related to s