fmr1基因敲除小鼠耳蜗形态学分析及其耳蜗 听觉皮层中gad gabaα1受体表达与ags的关系word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 表达随周龄增加逐渐减少，P0.01，差异具有显著性；不同周龄 WT 小鼠耳蜗及 听觉皮层 GAD 阳性细胞数表达无差异性，P〉0.05，差异无统计学意义。不同周 龄 KO 小鼠耳蜗 GABAα1 受体阳性细胞数表达无随年龄增加逐渐增加或减少趋 势，P〉0.05，差异无统计学意义；不同周龄 KO 小鼠听觉皮层 GABAα1 受体阳 性细胞数表达随周龄逐渐增加，P0.01，差异有统计学意义；不同周龄 WT 小鼠 耳蜗及听觉皮层 GABAα1 受体阳性细胞数表达无年龄差异性，P〉0.05，差异无 统计学意义。 【结论】 1． 本研究首次发现 FMR-1 基因敲除小鼠与 WT 小鼠耳蜗 HE 染色后观察形态结 构无明显差异。 2． 3、4、6 周各组 KO 小鼠的耳蜗、听觉皮层中 GAD 表达的平均阳性细胞数均 高于 WT 小鼠；8 周 KO 小鼠耳蜗、听觉皮层中 GAD 表达的平均阳性细胞数较 WT 小鼠比较结果无差异。 3． 3、4、6 周各组 KO 小鼠耳蜗、听觉皮层中 GABAα1 受体表达的平均阳性细 胞数均低于 WT 小鼠，8 周 KO 小鼠耳蜗、听觉皮层中 GABAα1 受体表达的平均 阳性细胞数较 WT 小鼠比较结果无差异。 4． FMR-1 KO 小鼠耳蜗、听觉皮层中 GAD 与 GABAα1 受体阳性细胞数表达具 有年龄依赖性，可能是 KO 鼠 AGS 原因之一。 【关键词】：脆性 X 综合征；Fmr-1 基因敲除小鼠;听源性惊厥；GAD; GABAα1 受体  The research of cochlea morphology in FMR1 gene Knock-out Mice and the relationship of AGS with the expression of GAD and GABAα1 receptor in cochlea and cortex auditory Postgraduate candidate: Du Na Tutor: Prof. Zhang jianguo Instructor: Associate Professor .Chen shengqiang Department of otolaryngology, the 2nd Affiliated Hospital of Guangzhou Medical College, Guangzhou (510260), China Abstract Background Fragile X syndrome (FXS) is caused by chromosomal abnormality, is one of the most common forms of inherited mental retardation,and the case rate is just second only to down syndrome . Fragile X syndrome (FXS) results from a trinucleotide repeat (CGG) expansion in the 5’untranslated region of the gene FMR1 and subsequent hypermethylation which lead to transcriptional silencing of FMR1 and loss of its encoded protein, the fragile X mental retardation protein (FMRP). The main symptoms of FXS include facial abnormality, macro-orchidism, mental retardation and high susceptibility to epilepsy.It is reported that epilepsy occurs in 20 to 25% of individuals with FXS and paroxysmal EEG abnormality presents in about 50% of prepubescent boys with FXS. It has been found that FMRP is a brain specific mRNA binding protein that suppresses translation of target mRNAs. However, the exact mechanism remains e