基于代谢组学技术的铜线树钻对枣疯病的抗性机理分析-analysis of resistance mechanism of copper wire drill to jujube witches broom disease based on metabolomics technology.docxVIP

AbstractJujubebelongstoRhamnaceaeZizyphus.Jujubeisoneofimportanteconomictreespeciesanddriedfruit,alsoisoneofthemostancientanduniquespeciesinourcountry,thereis8000yearsofcultivationhistory.Becauseitiseasiertomanage,earliertofruit, nutritiousandversatile,thereismorethan30countriesintroduceitandspreadittoallovertheworld.InChina,manyareasandprovinceshaveplantedJujubetreeexceptHeilongjiangprovince.InShanxi,Hebei,Shandong,Henan,Shaanxiprovince,jujubecultivationareaisverylarge,ithasalreadybecomeanimportantpartofagriculturalproduction,andplayedapositiveroletotheprosperityoftheruraleconomyandimprovementofpeopleslivingstandards.XishanJiaoZaoisAnhuiChizhoutraditionalspecialtyfruittreespecies,andJujubecultivationwasharmedbyJujubewitchesbroom.Earlyin1984,professorWangGuyuan ofAnhuiagriculturaluniversityhorticulturecollegeproposedtousepaliurushemsleyanusasstocktoresistJWB.overtheyears,ithasbeenconfirmedthatpaliurushemsleyanusas stockhastheabilitytoresistJWB.TodaythemethedisstillwidespreadusedinthejujubecultivationbecauseitcaneffectivelypreventtheJWB,andmakethetreesgrowmoreprosperous,fruitqualityimprove.NewshootsofpaliurushemsleyanusstockandXishanJiaoZaostockwereusedastestmaterials. Combinedwithcharacteristics oftheplant materialitself, newshootssamplesof paliurushemsleyanusvaccinationstockwithcontrolgroupandjujubestockvaccinationgroupwithcontrolgroupwererespectivelypreparedbyusingmetabonomicstechnologythroughmetabonomicsanalysisofJujubetreenewshoots’metabolites.Togetprocessedaqueous phaseaftergrinding, weighing,extraction, derivatizationinjujubenewtips. AccordingtoNISTandWileyDatabase,metabolicflowchangesofthesemetabolitesintheprocessof jujubegrowthand developmentweredetectedbyusing GC– MSforsampleanalysis,thenthemaindifferencesofmetabolitesbydifferenttreatmenwerecompared.TheseresearchworkwillprovidebasistoresearchandrevealtheJWBdiseaseresistancemechanism.Established themetabolicspectrumanalysistechnology platformoftheaqueousextractofChinesejujubenewtip,andanalyzedtheselectedsamp