交通相关细颗粒物对cem-6t细胞凋亡影响-effect of traffic-related fine particles on cem - 6t cell apoptosis.docxVIP

group(P0.05). Gray-scale ratio of Cyt-c protein was increased in 20(excluding 48h), 80, 320μg/mL PM2.5 compared to control group after 24h and 48h exposure. the differences were statistically significant(P0.05). Gray-scale ratios of Caspase-9 protein in 20, 80, 320μg/mL PM2.5 were higher than control group after 24h and 48h exposure, and the differences were statistically significant(P0.05). Gray-scale ratios of Caspase-8 protein were increased in 20,80,320μg/mL PM2.5 compared to control group after 24h and 48h exposure, and the differences were statistically significant(P0.05). Gray-scale ratios of Fas-L protein were increased in 20(excluding 48h), 80, 320μg/mL PM2.5 compared to control group after 24h and 48h exposure, and the differences were statistically significant(P0.05). Gray-scale ratio of TNF-α protein was increased in 20(excluding 48h), 80, 320μg/mL PM2.5 compared to control group after 24h and 48h exposure, and the difference was statistically significant(P0.05).Conclusions: Traffic related fine particulate can induce apoptosis of CEM-6T both via mitochondrial pathway and membrane death receptor pathway.Keywords: PM2.5; early apoptosis; necrosis; automobile exhaust; CEM-6T;主要缩略语对照英文简称英文全称中文全称PCDProgrammed Cell Death细胞程序死亡BSABovine Serum Albumin牛血清白蛋白MTTMethyl thiazolyl tetrazolium四甲基偶氮唑盐Rhodamine 1232-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methylester罗丹明 123EDTAEthylene diamine tetra-acetic acid乙二胺四乙酸DMSODimethyl sulfoxide二甲基亚砜FCMFlow cytometer流式细胞仪PBSPhosphate buffered saline磷酸盐缓冲液PIPropidium Iodide碘化丙啶EBEthidium bromide溴化乙锭TCATrichloroacetic acid三氯醋酸三 羟 甲 基 氨 基TrisTris(Hydroxymethyl)aminomethaneSDSDodecyl sulfate,sodium salt甲烷十 二 烷 基 磺 酸 钠APammonium perchlorate过硫酸胺N,N,N,N- 四 甲TEMEDN,N,N,N-Tetramethylethylenediamine基二乙胺DEPCDiethypyrocarbonate焦碳酸二乙酯NP-40NonidetP-40润湿剂 P-40TNFTumor Necrosis Factor肿瘤坏死因子学位论文独创性声明本人声明，所呈交的学位论文系在导师指导下本文独立完成的研究成果。文中任何引 用他人的成果，均已做出明确标注或得到许可。论文内容未包含法律意义上已属于他人的 任何形式的研究成果，也不包含本人已用于其他学位申请的论文或成果。与我一同工作的 同志对本研究所做的