姜黄素对小鼠急性脊髓损伤后后肢功能及nf-κb表达的影响-effects of curcumin on hind limb function and nf - κ b expression after acute spinal cord injury in mice.docxVIP

优秀毕业论文 精品参考文献资料 CATcatalase过氧化氢酶GSHglutathione还原型谷胱甘肽MTmetallothionein金属硫蛋白SUMOsmall ubiquitin-likemodifier小泛素样修饰蛋白EPEthyl pyruvate丙酮酸乙酯DNADeoxyribose NucleicAcid脱氧核糖核酸Cox-2cyclooxygenase-2环氧化酶-2DCM Dimethyl cardamom Ming 二甲基小豆蔻明 姜黄素对小鼠急性脊髓损伤后后肢功能及 NF-κB 表达的影响 中文摘要： 目的： 探讨姜黄素对小鼠急性脊髓损伤后后肢运动功能及 NF-κB 表达的影响，为临床 治疗急性脊髓损伤提供实验数据。 方法： 采用改良Allen’s法建立小鼠急性脊髓损伤的实验动物模型,将健康清洁级小鼠 随机分为损伤组（急性脊髓损伤组，A组）、干预组（脊髓损伤后进行姜黄素治疗 的干预组，B组）、对照组（单纯椎板摘除而无脊髓损伤组，C组），其中B组在损 伤后30min和12h时予姜黄素固体分散剂溶液（姜黄素200mg/kg）灌胃治疗干预， 而A、C组则在相同时相点予等量生理盐水灌胃。应用BMS评分体系、HE染色及 Western blot分别对各组小鼠脊髓损伤后神经功能、脊髓组织病理及脊髓组织内 NF-κBp65在各个时相点的表达进行研究。 结果： 1、神经功能学 BMS 评 分 ： 药 物 干 预 组 的 小 鼠 神 经 功 能 较 损 伤 组 显 著 改 善 （P0.05），但比对照组降低。 2、HE染色组织病理：药物干预组小鼠早期炎症细胞浸润相对较少，后期神经细 胞坏死减轻；而对照组未见明显炎症细胞浸润。 3、Western-blot检测：药物干预组有一定量NF-κBp65的表达，低于大量NF-κ Bp65表达的损伤组(P0.05)，但比对照组增高。 结论： 姜黄素干预后的急性脊髓损伤小鼠脊髓组织内NF-κBp65表达下降，炎症细胞浸 润减少，后肢神经功能有所改善，其在一定程度上能促进神经功能恢复。 关键词： 脊髓损伤,姜黄素,NF-κB,小鼠,后肢神经运动功能 Effect of the curcum on motor function of hind limbs and expression of NF-κB after acute spinal cord injured in mouses Abstract Objective: To explore the therapeutic potential of Curcum in a clinically relevant mouse model of contusion SCI, and to present experimental data for clinical treatment of acute SCI. Methods:The mouse SCI model was established with modified Allen’s method. The health clean level mouses were divided into three groups: the injury group(acute spinal cord injury group, A group), the treatment group(acute spinal cord injury with mini-dose curcum-treated group, B group) and the control group(simple vertebral plate removal without spinal cord injury group, C group). The mouses of group B were lavaged by curcum with the dose of 200 mg/kg after 30 min and 12 hours of injury,While the mouse of group A and C were treatde with saline lavage as control.The hindlimb locomotor recovery was evaluated by BMS score system. The spinal cord tissue pathology were observed by HE staining.The expression levels of NF-κBp65 were inv