DEN2 NGC株感染BALBc小鼠TH1TH2类细胞因子产生的差异①.docVIP

DEN2 NGC株感染BALB/c小鼠TH1/TH2类细胞因子产生的差异① 【摘要】目的：通过观察2型登革病毒（DEN2）NGC株初次感染和再次感染BALB/c小鼠后小鼠血浆中TH1和TH2类细胞因子的产生及其动态变化，研究DEN2感染的免疫机制。方法：用不同量的DEN2 NGC株经皮下多点注射，建立BALB/c小鼠感染模型，间接ELISA法测定感染后不同时间小鼠血浆IL2、IFNγ、IL4、IL5、 IL6、 IL10的含量。结果：（1）在初、再次感染阶段，DEN2 NGC株各感染组产生IL4、 IFNγ、 IL2、IL5、 IL6、 IL10与正常对照组有显著差异(P＜0.05)。①在初次感染阶段，NGC株各感染组血浆IL2、IL5、IL6于第4、6、8天出现；在再次感染阶段，各感染组产生IL2、IL5、IL6均于第1天迅速升高；IL2水平于第4天达高峰（104 448.46±13 314.1 pg/ml），维持5～7天后迅速下降；IL5于48小时达到高峰（135.125±46.75 pg/ml）；各组小鼠IL6产生的动态相似，在再次感染后第1天，达高峰（555.823±44.639 pg/ml）后逐渐下降。②初次感染早期，IL4水平明显升高，而IFNγ处于较低水平；再次感染后第1天，IL4水平达最高峰（4 294.668±349.038 pg/ml），IFNγ则于第4天和第11天达较高水平，两者的产生彼消此长，且产生动态与感染病毒量有关。（2）在初、再次感染阶段，用不同量DEN2 NGC株感染组产生细胞因子水平有差异。结论：DEN2 NGC株初次和再次感染BALB/c小鼠后，TH1和TH2应答均可发挥一定作用，二者相互影响， TH细胞及其产生的CK在DEN感染的发病机制中起重要作用。 【关键词】 登革2型病毒 TH1 TH2 细胞因子 The study for the dynamic levels of TH1/TH2 cytokines in the BALB/c mice infected with dengue type 2 virus NGC strain CHEN WenJie, ZUO Li, PAN Yu，SHANG ZhengLing. Department of Immunology，The Center of Tissue Engineering and Stem Cell Research, Guiyang Medical College, Guiyang 550004, China ［Abstract］ Objective:To observe the dynamic levels of TH1/TH2 cytokines from the plasma of BALB/c mice infected with DEN2 NGC strain and research immune response mechanism.Methods:The BALB/c mice plasma samples collected from different experiment groups at various time after infection were tested for IL2,IFNgamma;,IL4,IL5, IL6 and IL10 levels with indirect ELISA.Results:(1)The BALB/c mice could produce IL2,IFNgamma;,IL4,IL5,IL6 and IL10 after DEN2 infection and the correlation of cytokines with normal group(P＜0.05).①The cytokines that appeared were IL2 on the fourth day,IL5 on the sixth day,and IL6 on the eighth day after DEN2 infection;In the reinfection phase,IL2 appeared on the first day with a rapid peak on the fourth day(104 448.46±13 314.1 pg/ml) and then declined quickly after 5 to 6 days;IL5 appeared on the first day and reached a peak(135.125±46.75 pg/ml);I