PHARMACYFeb.2005DPC4SMAD4在子宫内膜癌中的表达及意义.docVIP

PHARMACYFeb.2005DPC4/SMAD4在子宫内膜癌中的表达及意义 【关键词】 子宫内膜癌 摘 要 目的 探讨Smad4蛋白在子宫内膜癌中的表达情况及其意义。 方法 应用免疫组织化学SP方法检测56例子宫内膜癌Smad4的表达情况，并与30例正常子宫内膜和42例各型增生性子宫内膜对比。结果 Smad4在子宫内膜癌中的表达明显低于正常子宫内膜和单纯性增生性子宫内膜（P＜0.01，P＜0.05），且与组织学分级及临床分期明显相关（P＜0.01，P＜0.05），而与肌层浸润深度无关。 结论 Smad4蛋白的表达可能在子宫内膜癌的发生发展及判断预后方面具有重要作用，可作为重要的生物学标记物。 关键词 Smad4蛋白；子宫内膜癌；免疫组织化学 Expression of Smad4 protein in endometrial carcinoma and its significance GE Xiujun，LIU Zhihui, LI Yingyong (1. Department of obstetrics and gynecology, Guangdong College of Pharmacy，Guangzhou 510240,China; 2. Department of clinical laboratory, Affiliated Hospital of Traditional Medical University of Guangzhou, Guangzhou 510405,China; 3. Department of obstetrics and gynecology, Affiliated Hospital of Guangdong Medical College，Guangdong 524023,China) Abstract Objective To investigate the expression of in endometrial carcinoma and its clinical significance. Methods Smad4 protein was detected in 56 endometrial carcinoma samples and 72 normal or/and benign hypertrophic endometrium samples by immunohistochemical SP method. Results Compared with normal controls and single hyperplasia, Smad4 protein was decreased in endometrial carcinoma tissue (P0.01, P0.05). The expression of Smad4 was correlated with histological grade and clinical stage (P0.01, P0.05)，while no significant difference was detected in tumors of different myometrial carcinoma samples. Conclusion Smad4 protein may play an important role in the development and prognosis of endometrial carcinoma. It may be used as an important biological marker in endometrial carcinoma. Key words Smad4 protein；endometrial carcinoma；immunohistochemistry 子宫内膜癌是女性生殖道常见的恶性肿瘤之一，近20年来，子宫内膜癌的发病率在全球范围内呈上升趋势，是最常见的妇科肿瘤。大约70%的子宫内膜癌在确诊时为早期病例，早期预后较好，但晚期预后较差，Ⅳ期患者5年生存率仅5%~15%［1］，因此积极探索子宫内膜癌的病理机制及准确判断预后已成为国内外学者广泛关注的问题，其编码的蛋白为SMAD4。DPC4 (deleted in pancreatic cancer locus 4)是新近克隆出的一种抑癌基因。研究表明，大约有55%的胰腺癌存在DPC4的缺失或失活，其中35%为纯合子丢失，20%为杂合子丢失［2］。但后来发现其主要作用是参与转化生长因子β （transforming growth factorbeta，TGFβ）超家族的信号转导，Smad4蛋白为TGFβ信号转导